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Title: Adipose mesenchymal stromal cells isolated from type 2 diabetic patients display reduced fibrinolytic activity.
Authors: Acosta, Lourdes
Hmadcha, Abdelkrim
Escacena, Natalia
Pérez-Camacho, Inmaculada
de la Cuesta, Antonio
Ruiz-Salmeron, Rafael
Gauthier, Benoit R
Soria, Bernat
metadata.dc.contributor.authoraffiliation: [Acosta,L; Hmadcha,A; Escacena,N; Gauthier,BR; Soria,B] Centro Andaluz de Biología Molecular y Medicina Regenerativa, Sevilla, Spain. [Hmadcha,A; Soria,B] CIBER de Diabetes y Enfermedades Metabolicas Asociadas, Barcelona, Spain. [Pérez-Camacho, I; de la Cuesta,A; Ruiz-Salmeron,R] Hospitales Universitarios San Lázaro and Virgen Macarena, Sevilla, Spain
Keywords: Tissue Plasminogen Activator;Plasminogen Activator Inhibitor 1;Células del Estroma Mesenquimatoso;Diabetes Mellitus, Type 2;Fibrinólisis
metadata.dc.subject.mesh: Medical Subject Headings::Named Groups::Persons::Age Groups::Adolescent
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over
Medical Subject Headings::Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus, Type 2
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Blood Physiological Phenomena::Blood Physiological Processes::Hemostasis::Blood Coagulation::Fibrinolysis
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Blood Coagulation Factors::Plasminogen Inactivators::Plasminogen Activator Inhibitor 1
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Blood Coagulation Factors::Tissue Plasminogen Activator
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue
Issue Date: Dec-2013
Publisher: American Diabetes Association
Citation: Acosta L, Hmadcha A, Escacena N, Pérez-Camacho I, de la Cuesta A, Ruiz-Salmeron R, et al. Adipose mesenchymal stromal cells isolated from type 2 diabetic patients display reduced fibrinolytic activity. Diabetes. 2013; 62(12):4266-9
Abstract: Stem cells have been successfully used for the treatment of critical limb ischemia (CLI). We conducted a clinical trial to determine the feasibility of using autologous adipose-derived mesenchymal stromal cells (AdMSCs) for the treatment of CLI. Unexpectedly, two diabetic patients developed peripheral microthrombosis. This adverse effect, which contrasts with the reported antithrombotic properties of MSCs, may stem from the diabetic environment that alters the fibrinolytic activity of AdMSCs, thereby increasing the probability of developing thrombosis. Here, we confirm this premise by demonstrating that diabetic AdMSCs cultured in the presence of blood sera expressed and released higher levels of plasminogen activator inhibitor type 1, reduced levels of tissue plasminogen activator, and lower d-dimer formation compared with nondiabetic AdMSCs. Thus, to establish an appropriate cell therapy for diabetic patients, we recommend including new preclinical safety tests, such as the d-dimer and/or the tissue plasminogen activator-to-plasminogen activator inhibitor type 1 ratio tests, to assess fibrinolytic activity of cells before implantation.
Description: Journal Article; Research Support, Non-U.S. Gov't;
metadata.dc.identifier.doi: 10.2337/db13-0896
ISSN: 1939-327X (Online)
0012-1797 (Print)
Appears in Collections:01- Artículos - CABIMER. Centro Andaluz de Biología Molecular
01- Artículos - Hospital Virgen Macarena

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