Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2264
Título : In1-ghrelin splicing variant is overexpressed in pituitary adenomas and increases their aggressive features.
Autor : Ibáñez-Costa, Alejandro
Gahete, Manuel D
Rivero-Cortés, Esther
Rincón-Fernández, David
Nelson, Richard
Beltrán, Manuel
de la Riva, Andrés
Japón, Miguel A
Venegas-Moreno, Eva
Gálvez, Ma Ángeles
García-Arnés, Juan A
Soto-Moreno, Alfonso
Morgan, Jennifer
Tsomaia, Natia
Culler, Michael D
Dieguez, Carlos
Castaño, Justo P
Luque, Raúl M
Filiación: [Ibáñez-Costa,A; Gahete,MD; Rivero-Cortés,E; Rincón-Fernández,D; Castaño,JP; Luque,RM] Department of Cell Biology, Physiology and Immunology, University of Cordoba, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario Reina Sofia; CIBER Fisiopatología de la Obesidad y Nutrición; and Campus de Excelencia Internacional Agroalimentario (ceiA3), Córdoba, Spain. [Nelson,ÇR; Morgan,J; Tsomaia,N; Culler,MD] IPSEN Bioscience, Cambridge, Massachusetts, USA. [Beltrán,M] Department of Pathology, Puerta del Mar University Hospital, Cádiz. [de la Riva,A] Service of Neurosurgery, Hospital Universitario Reina Sofia, Córdoba, Spain. [Japón,MA] Department of Pathology, Hospital Universitario Virgen del Rocío, Seville, Spain. [Venegas-Moreno,E; Soto-Moreno,A] Metabolism and Nutrition Unit, Hospital Universitario Virgen del Rocío, Instituto de Biomedicina de Sevilla (IBIS), Seville, Spain. [Gálvez,MA] Service of Endocrinology and Nutrition, Hospital Universitario Reina Sofia, and Instituto Maimónides de Investigación Biomédica de Córdoba, Córdoba, Spain. [García-Arnés,JA] Department of Endocrinology and Nutrition, Carlos Haya Hospital, Málaga, Spain. [Dieguez,C] Department of Physiology, University of Santiago de Compostela, and CIBER Fisiopatología de la Obesidad y Nutrición, Santiago de Compostela, Spain.
Palabras clave : Adenoma
Corticotropina
Empalme alternativo
Animales
Apoptosis
Inmunotransferencia Western
Células CHO
Línea celular tumoral
Cricetinae
Cinasas MAP reguladas por señales extracelulares
Regulación de la expresión génica neoplásica
Ghrelina
Hormona del crecimiento
Humanos
Intrones
Datos de Secuencia Molecular
Péptidos
Neoplasias hipofisarias
Isoformas de proteínas
Proteínas protooncogénicas c-akt
Interferencia por ARN
Reacción en cadena de la polimerasa por transcriptasa inversa
Transducción de señales
Células tumorales cultivadas
MeSH: Medical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Glandular and Epithelial::Adenoma
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pituitary Hormones::Pituitary Hormones, Anterior::Pro-Opiomelanocortin::Adrenocorticotropic Hormone
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::RNA Processing, Post-Transcriptional::RNA Splicing::Alternative Splicing
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Molecular Probe Techniques::Immunoassay::Immunoblotting::Blotting, Western
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::CHO Cells
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Cricetinae
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::Mitogen-Activated Protein Kinases::Extracellular Signal-Regulated MAP Kinases
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Gene Expression Regulation, Neoplastic
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Ghrelin
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pituitary Hormones::Pituitary Hormones, Anterior::Growth Hormone
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Gene Components::Introns
Medical Subject Headings::Information Science::Information Science::Information Services::Documentation::Molecular Sequence Data
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides
Medical Subject Headings::Diseases::Endocrine System Diseases::Pituitary Diseases::Pituitary Neoplasms
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Protein Isoforms
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Neoplasm Proteins::Oncogene Proteins::Proto-Oncogene Proteins::Proto-Oncogene Proteins c-akt
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Epigenesis, Genetic::Gene Silencing::RNA Interference
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Signal Transduction
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Tumor Cells, Cultured
Fecha de publicación : 4-Mar-2015
Editorial : Nature Publishing Group
Cita Bibliográfica: Ibáñez-Costa A, Gahete MD, Rivero-Cortés E, Rincón-Fernández D, Nelson R, Beltrán M, et al. In1-ghrelin splicing variant is overexpressed in pituitary adenomas and increases their aggressive features. Sci Rep. 2015; 5:8714
Abstract: Pituitary adenomas comprise a heterogeneous subset of pathologies causing serious comorbidities, which would benefit from identification of novel, common molecular/cellular biomarkers and therapeutic targets. The ghrelin system has been linked to development of certain endocrine-related cancers. Systematic analysis of the presence and functional implications of some components of the ghrelin system, including native ghrelin, receptors and the recently discovered splicing variant In1-ghrelin, in human normal pituitaries (n = 11) and pituitary adenomas (n = 169) revealed that expression pattern of ghrelin system suffers a clear alteration in pituitary adenomasas comparedwith normal pituitary, where In1-ghrelin is markedly overexpressed. Interestingly, in cultured pituitary adenoma cells In1-ghrelin treatment (acylated peptides at 100 nM; 24–72 h) increased GH and ACTH secretion, Ca2+ and ERK1/2 signaling and cell viability, whereas In1-ghrelin silencing (using a specific siRNA; 100 nM) reduced cell viability. These results indicate that an alteration of the ghrelin system, specially its In1-ghrelin variant, could contribute to pathogenesis of different pituitary adenomas types, and suggest that this variant and its related ghrelin system could provide new tools to identify novel, more general diagnostic, prognostic and potential therapeutic targets in pituitary tumors.
URI: http://hdl.handle.net/10668/2264
Versión del editor : http://www.nature.com/articles/srep08714#abstract
DOI: 10.1038/srep08714
ISSN : 2045-2322 (Online)
Appears in Collections:01- Artículos - IBIS. Instituto de Biomedicina de Sevilla
01- Artículos - Hospital Virgen del Rocío
01- Artículos - Hospital Puerta del Mar
01- Artículos - Hospital Reina Sofía
01- Artículos - IMIBIC. Instituto Maimónides de Investigación Biomédica de Córdoba
01- Artículos - Hospital Regional de Málaga

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