Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2281
Título : Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital.
Autor : Villalón, Pilar
Valdezate, Sylvia
Cabezas, Teresa
Ortega, Montserrat
Garrido, Noelia
Vindel, Ana
Medina-Pascual, María J
Saez-Nieto, Juan A
Filiación: [Villalón,P; Valdezate,S; Ortega,M; Garrido,N; Medina-Pascual,MJ; Saez-Nieto,JA] Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. [Cabezas,T] Laboratorio de Biotecnología Hospital de Poniente, El ejido, Almería, Spain. [Videl,A] Laboratorio de Infecciones intrahospitalarias Servicio de Bacteriología, Centro Nacional de Microbiología Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Palabras clave : A baumannii
PFGE
sequence type
Acinetobacter baumannii
MLVA
Genotipo
Clone
ADN Bacteriano
Tipificación de Secuencias Multilocus
MeSH: Medical Subject Headings::Organisms::Bacteria::Proteobacteria::Gammaproteobacteria::Moraxellaceae::Acinetobacter::Acinetobacter baumannii
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Epidemiology
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA::Multilocus Sequence Typing
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism, Genetic::Polymorphism, Restriction Fragment Length
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Moraxellaceae Infections::Acinetobacter Infections
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Tandem Repeat Sequences::Minisatellite Repeats
Fecha de publicación : 25-Feb-2015
Editorial : BioMed Central
Cita Bibliográfica: Villalón P, Valdezate S, Cabezas T, Ortega M, Garrido N, Vindel A, et al. Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital. BMC Microbiol. 2015; 15:47
Abstract: BACKGROUND Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period. RESULTS PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively. CONCLUSIONS PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/2281
Versión del editor : http://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-015-0383-y
DOI: 10.1186/s12866-015-0383-y
ISSN : 1471-2180 (Online)
Appears in Collections:01- Artículos - APES Hospital de Poniente

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