Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2299
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dc.contributor.authorMorata-Tarifa, Cynthia-
dc.contributor.authorJiménez, Gema-
dc.contributor.authorGarcía, María A-
dc.contributor.authorEntrena, José M-
dc.contributor.authorGriñán-Lisón, Carmen-
dc.contributor.authorAguilera, Margarita-
dc.contributor.authorPicon-Ruiz, Manuel-
dc.contributor.authorMarchal, Juan A-
dc.date.accessioned2016-08-05T08:14:55Z-
dc.date.available2016-08-05T08:14:55Z-
dc.date.issued2016-01-11-
dc.identifier.citationMorata-Tarifa C, Jiménez G, García MA, Entrena JM, Griñán-Lisón C, Aguilera M, et al. Low adherent cancer cell subpopulations are enriched in tumorigenic and metastatic epithelial-to-mesenchymal transition-induced cancer stem-like cells. Sci Rep. 2016; 6:18772es
dc.identifier.issn2045-2322 (Online)-
dc.identifier.otherPMC4707518-
dc.identifier.urihttp://hdl.handle.net/10668/2299-
dc.descriptionJournal Article;es
dc.description.abstractCancer stem cells are responsible for tumor progression, metastasis, therapy resistance and cancer recurrence, doing their identification and isolation of special relevance. Here we show that low adherent breast and colon cancer cells subpopulations have stem-like properties. Our results demonstrate that trypsin-sensitive (TS) breast and colon cancer cells subpopulations show increased ALDH activity, higher ability to exclude Hoechst 33342, enlarged proportion of cells with a cancer stem-like cell phenotype and are enriched in sphere- and colony-forming cells in vitro. Further studies in MDA-MB-231 breast cancer cells reveal that TS subpopulation expresses higher levels of SLUG, SNAIL, VIMENTIN and N-CADHERIN while show a lack of expression of E-CADHERIN and CLAUDIN, being this profile characteristic of the epithelial-to-mesenchymal transition (EMT). The TS subpopulation shows CXCL10, BMI-1 and OCT4 upregulation, differing also in the expression of several miRNAs involved in EMT and/or cell self-renewal such as miR-34a-5p, miR-34c-5p, miR-21-5p, miR-93-5p and miR-100-5p. Furthermore, in vivo studies in immunocompromised mice demonstrate that MDA-MB-231 TS cells form more and bigger xenograft tumors with shorter latency and have higher metastatic potential. In conclusion, this work presents a new, non-aggressive, easy, inexpensive and reproducible methodology to isolate prospectively cancer stem-like cells for subsequent biological and preclinical studies.es
dc.description.sponsorshipThis work was supported in part by grants from the Ministry of Economy and Competitiveness, Instituto de Salud Carlos III (FEDER funds, projects n°. PI10/02295 and PI10/02149), the Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía (Ministry of Health, Government of Andalucia, project number PI-0533-2014) and by Consejería Economía, Innovación, Ciencia y Empleo, Junta de Andalucia (Ministry of Economy, Innovation, Science and Employment, Government of Andalucia, excellence project number CTS-6568) providing a fellowship granted to GJ.es
dc.language.isoenes
dc.publisherNature Publishing Groupes
dc.relation.ispartofScientific reportses
dc.subjectAnimaleses
dc.subjectÍndice de masa corporales
dc.subjectMamaes
dc.subjectNeoplasias de la mamaes
dc.subjectCadherinases
dc.subjectClaudinases
dc.subjectNeoplasias del colones
dc.subjectTransición epitelial-mesenquimales
dc.subjectXenoinjertoses
dc.subjectRatoneses
dc.subjectRecurrencia local de neoplasiaes
dc.subjectCélulas madre neoplásicases
dc.subjectFenotipoes
dc.subjectTripsinaes
dc.subjectCaracoleses
dc.subjectRegulación hacia arribaes
dc.subjectVimentinaes
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animalses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Benzimidazoleses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Mass Indexes
dc.subject.meshMedical Subject Headings::Anatomy::Body Regions::Breastes
dc.subject.meshMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Breast Neoplasmses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Cell Adhesion Molecules::Cadherinses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Claudinses
dc.subject.meshMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Gastrointestinal Neoplasms::Intestinal Neoplasms::Colorectal Neoplasms::Colonic Neoplasmses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Transdifferentiation::Epithelial-Mesenchymal Transitiones
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Micees
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::MicroRNAses
dc.subject.meshMedical Subject Headings::Diseases::Neoplasms::Neoplastic Processes::Neoplasm Recurrence, Locales
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Stem Cells::Neoplastic Stem Cellses
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Phenotypees
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Mollusca::Gastropoda::Snailses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Trypsines
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Up-Regulationes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Intermediate Filament Proteins::Vimentines
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Transplants::Heterograftses
dc.titleLow adherent cancer cell subpopulations are enriched in tumorigenic and metastatic epithelial-to-mesenchymal transition-induced cancer stem-like cells.es
dc.typeinfo:eu-repo/semantics/articlees
dc.description.versionYeses
dc.identifier.pmid26752044-
dc.rights.accessRightsAcceso abiertoes
dc.identifier.doi10.1038/srep18772-
dc.type.versioninfo:eu-repo/semantics/publishedes
dc.relation.publisherversionhttp://www.nature.com/articles/srep18772#abstractes
dc.contributor.authoraffiliation[Morata-Tarifa,C; Jiménez,G; García,MA; Griñán-Lisón,C; Picon-Ruiz,M; Marchal,JA] Biopathology and Medicine Regenerative Institute (IBIMER), University of Granada, Granada, Spain. [Morata-Tarifa,C; Jiménez,G; García,MA; Griñán-Lisón,C; Aguilera,M; Picon-Ruiz,M; Marchal,JA] Biosanitary Institute of Granada (ibs.GRANADA), University Hospitals of Granada-Univesity of Granada, Granada, Spain. [Jiménez,G; Marchal,JA] Department of Human Anatomy and Embryology, University of Granada, Granada, Spain. [García,MA] Department of Oncology, University Hospital Virgen de las Nieves, Granada, Spain. [Entrena,JM] Institute of Neuroscience, Biomedical Research Center, University of Granada, Granada, Spain. Animal Behavior Research Unit, Scientific Instrumentation Center, University of Granada, Granada, Spain. [Aguilera,M] Department of Microbiology, University of Granada, Granada, Spain. [Picon-Ruiz,M] Braman Family Breast Cancer Institute, Sylvester Comprehensive Cancer Center, University of Miami, Miller School of Medicine, Miami, Florida, USA.es
dc.type.subtypeArtículoes
Appears in Collections:01- Artículos - ibsGRANADA. Instituto de Investigación Biosanitaria de Granada
01- Artículos - Complejo Hospitalario Universitario de Granada

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