Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2339
Título : Pharmacological activation of CB2 receptors counteracts the deleterious effect of ethanol on cell proliferation in the main neurogenic zones of the adult rat brain.
Autor : Rivera, Patricia
Blanco, Eduardo
Bindila, Laura
Alen, Francisco
Vargas, Antonio
Rubio, Leticia
Pavón, Francisco J
Serrano, Antonia
Lutz, Beat
Rodríguez de Fonseca, Fernando
Suárez, Juan
Filiación: [Rivera,P; Blanco,E; Alen,F; Vargas,A; Pavón,FJ; Serrano,A; Rodríguez de Fonseca,F; Suárez,J[ UGC Salud Mental, Instituto de Investigación Biomédica de Málaga, Universidad de Málaga-Hospital Universitario Regional de Málaga, Málaga, Spain. [Blanco,E] Departament de Pedagogia i Psicologia, Facultat de Ciències de l'Educació, Universitat de Lleida, Lleida, Spain. [Bindila,L; Lutz,B] Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg-University of Mainz, Mainz, Germany. [Rubio,L] Departamento de Anatomía y Medicina Legal, Universidad de Málaga, Málaga, Spain.
Palabras clave : Alcohol
ACEA
JWH133
CB1 receptor
Neurogenesis
CB2 receptor
Consumo de alcohol
Alcoholismo
Benzamidas
bromodesoxiuridina
Carbamatos
Giro dentado
Dieta
Endocannabinoides
Etanol
marcadores genéticos
Histonas
Hidrolasas
Hipotálamo
Ventrículos laterales
Células madre nerviosas
Neuronas
Fosforilación
Ratas
Receptor cannabinoide CB1
Sacarosa
Tubulina (proteína)
MeSH: Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Drinking Behavior::Alcohol Drinking
Medical Subject Headings::Psychiatry and Psychology::Mental Disorders::Substance-Related Disorders::Alcohol-Related Disorders::Alcoholism
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Benzamides
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleosides::Deoxyribonucleosides::Deoxyuridine::Bromodeoxyuridine
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Acids, Acyclic::Carbamates
Medical Subject Headings::Anatomy::Nervous System::Central Nervous System::Brain::Prosencephalon::Telencephalon::Cerebrum::Cerebral Cortex::Hippocampus::Dentate Gyrus
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Neurotransmitter Agents::Endocannabinoids
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Alcohols::Ethanol
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Phenotype::Genetic Markers
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nuclear Proteins::Histones
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases
Medical Subject Headings::Anatomy::Nervous System::Central Nervous System::Brain::Prosencephalon::Diencephalon::Hypothalamus
Medical Subject Headings::Anatomy::Nervous System::Central Nervous System::Brain::Cerebral Ventricles::Lateral Ventricles
Medical Subject Headings::Anatomy::Cells::Stem Cells::Neural Stem Cells
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Morphogenesis::Embryonic and Fetal Development::Organogenesis::Neurogenesis
Medical Subject Headings::Anatomy::Nervous System::Neurons
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Phosphorylation
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, G-Protein-Coupled::Receptors, Cannabinoid::Receptor, Cannabinoid, CB1
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, G-Protein-Coupled::Receptors, Cannabinoid::Receptor, Cannabinoid, CB2
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::Oligosaccharides::Disaccharides::Sucrose
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nerve Tissue Proteins::Tubulin
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Neurotransmitter Agents::Cannabinoid Receptor Modulators::Cannabinoid Receptor Agonists
Fecha de publicación : 29-Sep-2015
Editorial : Frontiers Media
Cita Bibliográfica: Rivera P, Blanco E, Bindila L, Alen F, Vargas A, Rubio L, et al. Pharmacological activation of CB2 receptors counteracts the deleterious effect of ethanol on cell proliferation in the main neurogenic zones of the adult rat brain. Front Cell Neurosci. 2015; 9:379
Abstract: Chronic alcohol exposure reduces endocannabinoid activity and disrupts adult neurogenesis in rodents, which results in structural and functional alterations. Cannabinoid receptor agonists promote adult neural progenitor cell (NPC) proliferation. We evaluated the protective effects of the selective CB1 receptor agonist ACEA, the selective CB2 receptor agonist JWH133 and the fatty-acid amide-hydrolase (FAAH) inhibitor URB597, which enhances endocannabinoid receptor activity, on NPC proliferation in rats with forced consumption of ethanol (10%) or sucrose liquid diets for 2 weeks. We performed immunohistochemical and stereological analyses of cells expressing the mitotic phosphorylation of histone-3 (phospho-H3+) and the replicating cell DNA marker 5-bromo-2'-deoxyuridine (BrdU+) in the main neurogenic zones of adult brain: subgranular zone of dentate gyrus (SGZ), subventricular zone of lateral ventricles (SVZ) and hypothalamus. Animals were allowed ad libitum ethanol intake (7.3 ± 1.1 g/kg/day) after a controlled isocaloric pair-feeding period of sucrose and alcoholic diets. Alcohol intake reduced the number of BrdU+ cells in SGZ, SVZ, and hypothalamus. The treatments (URB597, ACEA, JWH133) exerted a differential increase in alcohol consumption over time, but JWH133 specifically counteracted the deleterious effect of ethanol on NPC proliferation in the SVZ and SGZ, and ACEA reversed this effect in the SGZ only. JWH133 also induced an increased number of BrdU+ cells expressing neuron-specific β3-tubulin in the SVZ and SGZ. These results indicated that the specific activation of CB2 receptors rescued alcohol-induced impaired NPC proliferation, which is a potential clinical interest for the risk of neural damage in alcohol dependence.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/2339
Versión del editor : http://journal.frontiersin.org/article/10.3389/fncel.2015.00379/full
DOI: 10.3389/fncel.2015.00379
ISSN : 1662-5102 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga

Files in This Item:
File Description SizeFormat 
RiveraP_PharmacologicalActivation.pdfArtículo publicado5,4 MBAdobe PDFView/Open
RiveraP_PharmacologicalActivation_DataSheet1.docDataSheet137 kBMicrosoft WordView/Open
RiveraP_PharmacologicalActivation_Image1.tiff.tifImage11,08 MBTIFFView/Open


This item is licensed under a Creative Commons License Creative Commons