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Title: Impaired B cells survival upon production of inflammatory cytokines by HIV-1 exposed follicular dendritic cells.
Authors: Sabri, Farideh
Prados, Alejandro
Muñoz-Fernández, Raquel
Lantto, Rebecka
Fernandez-Rubio, Pablo
Nasi, Aikaterini
Amu, Sylvie
Albert, Jan
Garcia Olivares, Enrique
Chiodi, Francesca
metadata.dc.contributor.authoraffiliation: [Sabri,F; Lantto,R; Nasi,A; Amu,S; Albert,J; Chiodi,F] Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. [Prados,A; Muñoz-Fernández,R; Fernandez-Rubio,P; Garcia Olivares,E] Unidad de Immunologia, Instituto de Biopatologia y Medicina Regenerativa, Complejo Hospitalario Universitario de Granada, Granada, Spain. [Albert,J] Department of Clinical Microbiology, Karolinska University, Hospital, Stockholm, Sweden.
Keywords: Follicular dendritic cells;HIV-1;Chemokines;Inflammation;B cells;Presentación de antígeno;Linfocitos B;Línea celular;Supervivencia celular;Citocinas;Células dendríticas foliculares;Genoma viral;Centro germinal;VIH-1;Tejido linfoide;Receptores del VIH
metadata.dc.subject.mesh: Medical Subject Headings::Phenomena and Processes::Immune System Phenomena::Immune System Processes::Antigen Presentation
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::B-Lymphocytes
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines
Medical Subject Headings::Anatomy::Cells::Antigen-Presenting Cells::Dendritic Cells, Follicular
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome, Viral
Medical Subject Headings::Anatomy::Hemic and Immune Systems::Immune System::Lymphatic System::Lymphoid Tissue::Germinal Center
Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Virus::Receptors, HIV
Medical Subject Headings::Organisms::Viruses::Vertebrate Viruses::RNA Viruses::Retroviridae::Lentivirus::Lentiviruses, Primate::HIV::HIV-1
Issue Date: 5-Sep-2016
Publisher: BioMed Central
Citation: Sabri F, Prados A, Muñoz-Fernández R, Lantto R, Fernandez-Rubio P, Nasi A, et al. Impaired B cells survival upon production of inflammatory cytokines by HIV-1 exposed follicular dendritic cells. Retrovirology. 2016; 13(1):61
Abstract: BACKGROUND Follicular dendritic cells (FDCs) are important components in the organization of germinal centers in lymphoid tissue where, following antigen presentation, B cells differentiate into memory B cells. The possibility of establishing primary cell lines from FDCs isolated from lymphoid tissue paved the way for characterization of FDC biological properties. We exposed primary FDC cell lines to HIV-1 strains in vitro and studied changes in the chemo-attractive properties of FDCs and release of inflammatory cytokines. RESULTS FDC lines expressed several known and putative HIV-1 receptors; viral genome was amplified in HIV-1 exposed FDCs which released low levels of p24 HIV-1 protein in culture supernatants, but were not definitely proven to be productively infected. Exposure of FDCs to HIV-1 strains did not change the expression of markers used to characterize these cells. HIV-1 exposed FDCs, however, changed the expression of chemo-attractants involved in cell recruitment at inflammatory sites and increased the production of several inflammatory cytokines. The inflammatory milieu created upon HIV-1 exposure of FDCs led to impaired B cell survival in vitro and reduced Ig production. CONCLUSIONS FDC lines exposed to different HIV-1 strains, although not able to support productive HIV-1 replication, show an increased production of inflammatory cytokines. Our in vitro model of interactions between HIV-1 exposed FDC lines and B cells suggest that exposure of FDCs to HIV-1 in vivo can contribute to inflammation within germinal centers and that this pathological event may impair B cell survival and contribute to impaired B cell responses during HIV-1 infection.
Description: Journal Article;
metadata.dc.identifier.doi: 10.1186/s12977-016-0295-4
ISSN: 1742-4690 (Online)
Appears in Collections:01- Artículos - Complejo Hospitalario Universitario de Granada

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