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dc.contributor.authorPérez-Parra, Santiago-
dc.contributor.authorChueca, Natalia-
dc.contributor.authorÁlvarez, Marta-
dc.contributor.authorPasquau, Juan-
dc.contributor.authorOmar, Mohamed-
dc.contributor.authorCollado, Antonio-
dc.contributor.authorVinuesa, David-
dc.contributor.authorLozano, Ana B-
dc.contributor.authorYebra, Gonzalo-
dc.contributor.authorGarcía, Federico-
dc.identifier.citationPérez-Parra S, Chueca N, Álvarez M, Pasquau J, Omar M, Collado A, et al. Phylodynamic and Phylogeographic Profiles of Subtype B HIV-1 Epidemics in South Spain. PLoS ONE. 2016; 11(12):e0168099es
dc.identifier.issn1932-6203 (Online)-
dc.descriptionJournal Article;es_ES
dc.description.abstractBACKGROUND Since 1982, HIV-1 epidemics have evolved to different scenarios in terms of transmission routes, subtype distribution and characteristics of transmission clusters. We investigated the evolutionary history of HIV-1 subtype B in south Spain. PATIENTS & METHODS We studied all newly diagnosed HIV-1 subtype B patients in East Andalusia during the 2005-2012 period. For the analysis, we used the reverse transcriptase and protease sequences from baseline resistance, and the Trugene® HIV Genotyping kit (Siemens, Barcelona, Spain). Subtyping was done with REGA v3.0. The maximum likelihood trees constructed with RAxML were used to study HIV-1 clustering. Phylogeographic and phylodynamic profiles were studied by Bayesian inference methods with BEAST v1.7.5 and SPREAD v1.0.6. RESULTS Of the 493 patients infected with HIV-1 subtype B, 234 grouped into 55 clusters, most of which were small (44 clusters ≤ 5 patients, 31 with 2 patients, 13 with 3). The rest (133/234) were grouped into 11 clusters with ≥ 5 patients, and most (82%, 109/133) were men who have sex with men (MSM) grouped into 8 clusters. The association with clusters was more frequent in Spanish (p = 0.02) men (p< 0.001), MSM (p<0.001) younger than 35 years (p = 0.001) and with a CD4+ T-cell count above 350 cells/ul (p<0.001). We estimated the date of HIV-1 subtype B regional epidemic diversification around 1970 (95% CI: 1965-1987), with an evolutionary rate of 2.4 (95%CI: 1.7-3.1) x 10-3 substitutions/site/year. Most clusters originated in the 1990s in MSMs. We observed exponential subtype B HIV-1 growth in 1980-1990 and 2005-2008. The most significant migration routes for subtype B went from inland cities to seaside locations. CONCLUSIONS We provide the first data on the phylodynamic and phylogeographic profiles of HIV-1 subtype B in south Spain. Our findings of transmission clustering among MSMs should alert healthcare managers to enhance preventive measures in this risk group in order to prevent future outbreaks.es_ES
dc.description.sponsorshipFunded by National Plan of I+D+i (RD12/0017/006), Health institute Carlos III(ISCIII), PI12/01053 (General Ministry of Evaluation and European Fund of Regional Development-FEDER), granted to FGG. Ministry of Health of Andalusia (AC-0082-2013), granted to FGG.es_ES
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPloS onees
dc.subjectLinfocitos T CD4-positivoses_ES
dc.subjectAnálisis por conglomeradoses_ES
dc.subjectInfecciones por VIHes_ES
dc.subjectHomosexualidad masculinaes_ES
dc.subjectPéptido hidrolasases_ES
dc.subjectADN polimerasa dirigida por ARNes_ES
dc.subjectTeorema de bayeses_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Bayes Theoremes_ES
dc.subject.meshMedical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes::CD4-Positive T-Lymphocyteses_ES
dc.subject.meshMedical Subject Headings::Geographicals::Geographic Locations::Citieses_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Cluster Analysises_ES
dc.subject.meshMedical Subject Headings::Health Care::Environment and Public Health::Public Health::Disease Outbreaks::Epidemicses_ES
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotypees_ES
dc.subject.meshMedical Subject Headings::Diseases::Virus Diseases::RNA Virus Infections::Retroviridae Infections::Lentivirus Infections::HIV Infectionses_ES
dc.subject.meshMedical Subject Headings::Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus::Lentiviruses, Primate::HIVes_ES
dc.subject.meshMedical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Sexual Behavior::Sexuality::Homosexuality::Homosexuality, Malees_ES
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses_ES
dc.subject.meshMedical Subject Headings::Check Tags::Malees_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolaseses_ES
dc.subject.meshMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Genetics::Genetics, Population::Phylogeographyes_ES
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probabilityes_ES
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Nucleotidyltransferases::DNA Nucleotidyltransferases::DNA-Directed DNA Polymerase::RNA-Directed DNA Polymerasees_ES
dc.subject.meshMedical Subject Headings::Geographicals::Geographic Locations::Europe::Spaines_ES
dc.titlePhylodynamic and Phylogeographic Profiles of Subtype B HIV-1 Epidemics in South
dc.rights.accessRightsAcceso abiertoes_ES
dc.contributor.authoraffiliation[Pérez-Parra,S; Chueca,N; Álvarez,M; García,F ] Servicio de Microbiología Clínica, Hospital Universitario San Cecilio, Complejo Hospitalario e Instituto de Investigación IBS, Granada, Spain. [Pasquau,J] Servicio de Infecciosas, Hospital Virgen de las Nieves, Granada, Spain. [Omar,M] Servicio de Infecciosas, Hospital Ciudad de Jaén, Jaén, Spain. [Collado,A] Servicio de Medicina Interna, Hospital de Torrecárdenas, Almería, Spain. [Vinuesa,D] Servicio de Infecciosas, Hospital Universitario San Cecilio, Granada, Spain. [Lozano,AB] Servicio de Infecciosas, Hospital de Poniente, Almería, Spain. [Yebra,G] Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United
Appears in Collections:01- Artículos - APES Hospital de Poniente
01- Artículos - Complejo Hospitalario Torrecárdenas
01- Artículos - Complejo Hospitalario de Jaén
01- Artículos - ibsGRANADA. Instituto de Investigación Biosanitaria de Granada
01- Artículos - Complejo Hospitalario Universitario de Granada

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