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Title: Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability.
Authors: Gouveia, Ricardo M
González-Andrades, Elena
Cardona, Juan C
González-Gallardo, Carmen
Ionescu, Ana M
Garzon, Ingrid
Alaminos, Miguel
González-Andrades, Miguel
Connon, Che J
metadata.dc.contributor.authoraffiliation: [Gouveia,R; Connon,CJ] Institute of Genetic Medicine, Newcastle University, International Centre for Life, Newcastle-upon-Tyne, UK. [González-Andrades,E; Garzon,I; Alaminos,M] Tissue Engineering Group, Department of Histology, Faculty of Medicine and Dentistry, University of Granada, Granada, Spain. [Cardona,JC; Ionescu,AM] Laboratory of Biomaterials and Optics, Optics Department, Faculty of Sciences, University of Granada, Granada, Spain. [González-Gallardo,C] San Cecilio University Hospital, Ophthalmology Service, Granada, Spain. [González-Andrades,M] Schepens Eye Research Institute and Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
Keywords: Tissue templating;Corneal stroma;SLATEs;Animales;Anisotropía;Materiales biocompatibles;Colágeno;Córnea;Sustancia propia;Epitelio anterior;Matriz extracelular;Estudios de seguimiento;Humanos;Inflamación;Elevación;Péptidos;Conejos;Células del Estroma
metadata.dc.subject.mesh: Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Optical Phenomena::Anisotropy
Medical Subject Headings::Chemicals and Drugs::Biomedical and Dental Materials::Biocompatible Materials
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen
Medical Subject Headings::Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea
Medical Subject Headings::Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea::Corneal Stroma
Medical Subject Headings::Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea::Epithelium, Corneal
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Extracellular Space::Extracellular Matrix
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Follow-Up Studies
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Mechanical Processes::Lifting
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Lagomorpha::Rabbits
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells
Issue Date: Mar-2017
Publisher: Elsevier
Citation: Gouveia RM, González-Andrades E, Cardona JC, González-Gallardo C, Ionescu AM, Garzon I, et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials. 2017 ; 121:205-219
Abstract: Ideally, biomaterials designed to play specific physical and physiological roles in vivo should comprise components and microarchitectures analogous to those of the native tissues they intend to replace. For that, implantable biomaterials need to be carefully designed to have the correct structural and compositional properties, which consequently impart their bio-function. In this study, we showed that the control of such properties can be defined from the bottom-up, using smart surface templates to modulate the structure, composition, and bio-mechanics of human transplantable tissues. Using multi-functional peptide amphiphile-coated surfaces with different anisotropies, we were able to control the phenotype of corneal stromal cells and instruct them to fabricate self-lifting tissues that closely emulated the native stromal lamellae of the human cornea. The type and arrangement of the extracellular matrix comprising these corneal stromal Self-Lifting Analogous Tissue Equivalents (SLATEs) were then evaluated in detail, and was shown to correlate with tissue function. Specifically, SLATEs comprising aligned collagen fibrils were shown to be significantly thicker, denser, and more resistant to proteolytic degradation compared to SLATEs formed with randomly-oriented constituents. In addition, SLATEs were highly transparent while providing increased absorption to near-UV radiation. Importantly, corneal stromal SLATEs were capable of constituting tissues with a higher-order complexity, either by creating thicker tissues through stacking or by serving as substrate to support a fully-differentiated, stratified corneal epithelium. SLATEs were also deemed safe as implants in a rabbit corneal model, being capable of integrating with the surrounding host tissue without provoking inflammation, neo-vascularization, or any other signs of rejection after a 9-months follow-up. This work thus paves the way for the de novo bio-fabrication of easy-retrievable, scaffold-free human tissues with controlled structural, compositional, and functional properties to replace corneal, as well as other, tissues.
Description: Journal Article;
metadata.dc.identifier.doi: 10.1016/j.biomaterials.2016.12.023
ISSN: 1878-5905 (Online)
0142-9612 (Print)
Appears in Collections:01- Artículos - Complejo Hospitalario Universitario de Granada

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