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Title: | The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice |
Authors: | Fernández-Caballero, J A Alvarez, M Chueca, N Pérez, A B García, F |
metadata.dc.contributor.authoraffiliation: | Servicio de Microbiología, Complejo Hospitalario Universitario Granada-Hospital PTS, Instituto de Investigación Biosanitaria IBS, Granada, Spain. |
Keywords: | Secuencia de bases;Genotipo;Humanos;Patología molecular;Filogenia;Análisis de secuencia de ADN |
metadata.dc.subject.mesh: | Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Base Sequence Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype Medical Subject Headings::Diseases::Digestive System Diseases::Liver Diseases::Hepatitis::Hepatitis, Viral, Human::Hepatitis C Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans Medical Subject Headings::Disciplines and Occupations::Health Occupations::Medicine::Pathology::Pathology, Molecular Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Phylogeny Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA |
Issue Date: | 14-Apr-2017 |
Publisher: | Public Library of Science |
Citation: | Fernández-Caballero JA, Alvarez M, Chueca N, Pérez AB, García F. The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice. PLoS One. 2017 Apr 14;12(4):e0175564. |
Abstract: | OBJECTIVE: We aimed to evaluate the correct assignment of HCV genotype/subtypes 1a and 1b by cobas® HCV genotyping (GT) assay (Roche Molecular Diagnostics) compared with nonstructural protein 5B (NS5B) sequencing. PATIENTS AND METHODS: Clinical samples from 153 patients submitted for HCV genotyping were studied. After genotyping with the cobas® HCV GT, sequencing of a 387 bp fragment in the NS5B gene and phylogenetic analysis was employed to compare genotyping results. Major discrepancies were defined as differences in the assigned genotype by cobas® HCV GT and NS5B sequencing (including genotype 1 subtypes 1a and 1b misclassification). RESULTS: Overall agreement between the cobas® HCV GT and NS5B sequencing was 98%; all the 1a, 1b, 2, 3 and 4 genotypes identified by cobas® HCV GT were concordant with NS5B sequencing. Three samples tested "indetermined" by cobas® HCV GT assay and were genotyped as 1a, 3a, and 4d by NS5B sequencing. CONCLUSSION: These results indicate that the cobas® HCV GT assay correctly identifies HCV genotypes, and points out the importance of additional methods based on DNA sequencing for resolving indeterminate results. |
URI: | http://hdl.handle.net/10668/2642 |
metadata.dc.relation.publisherversion: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175564 |
metadata.dc.identifier.doi: | 10.1371/journal.pone.0175564 |
ISSN: | 1932-6203 (Online) |
Appears in Collections: | 01- Artículos - Complejo Hospitalario Universitario de Granada 01- Artículos - ibsGRANADA. Instituto de Investigación Biosanitaria de Granada |
Files in This Item:
File | Description | Size | Format | |
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FernandezCaballero_TheCobas®HCVGT.pdf | Artículo publicado | 417,92 kB | Adobe PDF | View/Open |
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