Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2642
Título : The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice
Autor : Fernández-Caballero, J A
Alvarez, M
Chueca, N
Pérez, A B
García, F
Filiación: Servicio de Microbiología, Complejo Hospitalario Universitario Granada-Hospital PTS, Instituto de Investigación Biosanitaria IBS, Granada, Spain.
Palabras clave : Secuencia de bases
Genotipo
Humanos
Patología molecular
Filogenia
Análisis de secuencia de ADN
MeSH: Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Base Sequence
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype
Medical Subject Headings::Diseases::Digestive System Diseases::Liver Diseases::Hepatitis::Hepatitis, Viral, Human::Hepatitis C
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Disciplines and Occupations::Health Occupations::Medicine::Pathology::Pathology, Molecular
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Phylogeny
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA
Fecha de publicación : 14-Apr-2017
Editorial : Public Library of Science
Cita Bibliográfica: Fernández-Caballero JA, Alvarez M, Chueca N, Pérez AB, García F. The cobas® HCV GT is a new tool that accurately identifies Hepatitis C virus genotypes for clinical practice. PLoS One. 2017 Apr 14;12(4):e0175564.
Abstract: OBJECTIVE: We aimed to evaluate the correct assignment of HCV genotype/subtypes 1a and 1b by cobas® HCV genotyping (GT) assay (Roche Molecular Diagnostics) compared with nonstructural protein 5B (NS5B) sequencing. PATIENTS AND METHODS: Clinical samples from 153 patients submitted for HCV genotyping were studied. After genotyping with the cobas® HCV GT, sequencing of a 387 bp fragment in the NS5B gene and phylogenetic analysis was employed to compare genotyping results. Major discrepancies were defined as differences in the assigned genotype by cobas® HCV GT and NS5B sequencing (including genotype 1 subtypes 1a and 1b misclassification). RESULTS: Overall agreement between the cobas® HCV GT and NS5B sequencing was 98%; all the 1a, 1b, 2, 3 and 4 genotypes identified by cobas® HCV GT were concordant with NS5B sequencing. Three samples tested "indetermined" by cobas® HCV GT assay and were genotyped as 1a, 3a, and 4d by NS5B sequencing. CONCLUSSION: These results indicate that the cobas® HCV GT assay correctly identifies HCV genotypes, and points out the importance of additional methods based on DNA sequencing for resolving indeterminate results.
URI: http://hdl.handle.net/10668/2642
Versión del editor : http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175564
DOI: 10.1371/journal.pone.0175564
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - ibsGRANADA. Instituto de Investigación Biosanitaria de Granada
01- Artículos - Complejo Hospitalario Universitario de Granada

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