Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2726
Título : Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients.
Autor : Comino, Isabel
Fernández-Bañares, Fernando
Esteve, María
Ortigosa, Luís
Castillejo, Gemma
Fambuena, Blanca
Ribes-Koninckx, Carmen
Sierra, Carlos
Rodríguez-Herrera, Alfonso
Salazar, José Carlos
Caunedo, Ángel
Marugán-Miguelsanz, J M
Garrote, José Antonio
Vivas, Santiago
Lo Iacono, Oreste
Nuñez, Alejandro
Vaquero, Luis
Vegas, Ana María
Crespo, Laura
Fernández-Salazar, Luis
Arranz, Eduardo
Jiménez-García, Victoria Alejandra
Antonio Montes-Cano, Marco
Espín, Beatriz
Galera, Ana
Valverde, Justo
Girón, Francisco José
Bolonio, Miguel
Millán, Antonio
Martínez Cerezo, Francesc
Guajardo, César
Alberto, José Ramón
Rosinach, Mercé
Segura, Verónica
León, Francisco
Marinich, Jorge
Muñoz-Suano, Alba
Romero-Gómez, Manuel
Cebolla, Ángel
Sousa, Carolina
Filiación: [Comino,I; Segura,V; Sousa,C] Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain. [Fernández-Bañares,F; Esteve,M; Rosinach,M] Department of Gastroenterology, Hospital Universitari Mutua Terrassa, and CIBERehd, Terrassa, Barcelona, Spain. [Ortigosa,L; Guajardo,C; Alberto, JR] Pediatric Gastroenterology, Hospital Universitario Nuestra Señora de La Candelaria, Tenerife, Spain. [Castillejo,G; Martínez Cerezo,F] Pediatric Gastroenterology, Hospital Universitari de Sant Joan de Reus, IISPV, URV, Reus, Spain. [Fambuena,B; Romero-Gómez,M; Millán,A] Unit for the Clinical Management of Digestive Diseases and CIBERehd and Gastroenterology and Nutrition Unit, Hospital Universitario Virgen de Valme, Seville, Spain. [Ribes-Koninckx,C; Bolonio,M] Pediatric Gastroenterology, Hepatology and Nutrition Unit, Hospital Universitario y Politécnico La Fe, Celiac Disease and Digestive Inmunopatology Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. [Sierra,C; Girón,.FJ] Pediatric Gastroenterology and Nutrition Unit, Hospital Materno-Infantil, Malaga, Spain. [Rodríguez-Herrera,A; Galera,A; Valverde,J] Gastroenterology and Nutrition Unit, Instituto Hispalense de Pediatría, Seville, Spain. [Salazar,JC; Espín,B] Servicio de Gastroenterología Pediátrica, Hospital Universitario Virgen del Rocío, Seville, Spain. [Caunedo,A; Jiménez-García,VA] Hospital Universitario Virgen Macarena, Seville, Spain. [Marugán-MiguelSanz,JM; Fernández-Salazar,L; Arranz,E] Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, CSIC and Gastroenterology Unit, Hospital Clínico Universitario de Valladolid, Valladolid, Spain. [Garrote,JA; Vegas, AM; Crespo,L] Clinical Analysis and Pediatrics, Hospital Universitario Río Hortega, Valladolid, Spain. [Vivas,S; Nuñez,A; Vaquero,LServicio de Aparato Digestivo, Hospital Universitario de Leon, Leon, Spain. [Lo Iacono,O] Sección de Aparato Digestivo, Hospital del Tajo, Madrid, Spain. [Montes-Cano,MA] Servicio de Inmunología, CIBER de Epidemiología y Salud Pública, Hospital Universitario Virgen del Rocío/IBiS/CSIC/Universidad de Sevilla, Seville, Spain. [León,F] Celimmune, Bethesda, Maryland, USA. [Marinich,J; Muñoz-Suano,A; Cebolla,A] Biomedal SL, Seville, Spain.
Palabras clave : Enfermedad celíaca
Dieta sin gluten
Ensayo de inmunoadsorción enzimática
Proteínas de unión al GTP
Gliadina
Glútenes
Humanos
Inmunoglobulina A
Masculino
Cooperación del paciente
Péptidos
Estudios prospectivos
Encuestas y cuestionarios
Transglutaminasas
MeSH: Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Malabsorption Syndromes::Celiac Disease
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet, Gluten-Free
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunohistochemistry::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assay
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::GTP-Binding Proteins
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutens::Gliadin
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutens
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin A
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Health Care::Health Care Quality, Access, and Evaluation::Delivery of Health Care::Attitude to Health::Patient Acceptance of Health Care::Patient Compliance
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides
Medical Subject Headings::Health Care::Health Care Quality, Access, and Evaluation::Quality of Health Care::Health Care Evaluation Mechanisms::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Prospective Studies
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Surveys and Questionnaires
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Acyltransferases::Aminoacyltransferases::Transglutaminases
Fecha de publicación : 11-Oct-2016
Editorial : Nature Publishing Group
Cita Bibliográfica: Comino I, Fernández-Bañares F, Esteve M, Ortigosa L, Castillejo G, Fambuena B, et al. Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients. Am. J. Gastroenterol.. 2016 ; 111(10):1456-1465
Abstract: OBJECTIVES Treatment for celiac disease (CD) is a lifelong strict gluten-free diet (GFD). Patients should be followed-up with dietary interviews and serology as CD markers to ensure adherence to the diet. However, none of these methods offer an accurate measure of dietary compliance. Our aim was to evaluate the measurement of gluten immunogenic peptides (GIP) in stools as a marker of GFD adherence in CD patients and compare it with traditional methods of GFD monitoring. METHODS We performed a prospective, nonrandomized, multicenter study including 188 CD patients on GFD and 84 healthy controls. Subjects were given a dietary questionnaire and fecal GIP quantified by enzyme-linked immunosorbent assay (ELISA). Serological anti-tissue transglutaminase (anti-tTG) IgA and anti-deamidated gliadin peptide (anti-DGP) IgA antibodies were measured simultaneously. RESULTS Of the 188 celiac patients, 56 (29.8%) had detectable GIP levels in stools. There was significant association between age and GIP in stools that revealed increasing dietary transgressions with advancing age (39.2% in subjects ≥13 years old) and with gender in certain age groups (60% in men ≥13 years old). No association was found between fecal GIP and dietary questionnaire or anti-tTG antibodies. However, association was detected between GIP and anti-DGP antibodies, although 46 of the 53 GIP stool-positive patients were negative for anti-DGP. CONCLUSIONS Detection of gluten peptides in stools reveals limitations of traditional methods for monitoring GFD in celiac patients. The GIP ELISA enables direct and quantitative assessment of gluten exposure early after ingestion and could aid in the diagnosis and clinical management of nonresponsive CD and refractory CD. Trial registration number NCT02711397.
Descripción : JOURNAL ARTICLE;
URI: http://hdl.handle.net/10668/2726
Versión del editor : http://www.nature.com/ajg/journal/v111/n10/full/ajg2016439a.html#abs
DOI: 10.1038/ajg.2016.439
ISSN : 1572-0241 (Online)
0002-9270 (Print)
Appears in Collections:01- Artículos - IBIS. Instituto de Biomedicina de Sevilla
01- Artículos - Hospital Virgen Macarena
01- Artículos - Hospital Virgen del Rocío
01- Artículos - Hospital de Valme
01- Artículos - Hospital Regional de Málaga

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