Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/3162
Title: Association of breast and gut microbiota dysbiosis and the risk of breast cancer: a case-control clinical study
Authors: Plaza-Díaz, Julio
Álvarez-Mercado, Ana I.
Ruiz-Marín, Carmen M.
Reina-Pérez, Iris
Pérez-Alonso, Alejandro J.
Sánchez-Andujar, María Belén
Torné, Pablo
Gallart-Aragón, Tania
Sánchez-Barrón, María Teresa
Reyes Lartategui, Saturnino
García, Federico
Chueca, Natalia
Moreno-Delgado, Ana
Torres-Martínez, Katia
Sáez-Lara, María José
Robles-Sánchez, Cándido
Fernández, Mariana F.
Fontana, Luis
metadata.dc.contributor.authoraffiliation: [Plaza-Díaz,J; Álvarez-Mercado,AI; Robles-Sánchez,C; Fontana,L] Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071, Granada, Spain. [Plaza-Díaz,J; Álvarez-Mercado,AI; Sáez-Lara,MJ; Robles-Sánchez,C; Fontana,L] Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Granada, Spain. [Plaza-Díaz,J; García,F; Chueca,N; Robles-Sánchez,C; Fernández,MF; Fontana,L] Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain. [Plaza-Díaz,J] Spanish Consortium for Research on Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. [Ruiz-Marín,CM; Pérez-Alonso,AJ; Sánchez-Andujar,MB] Unit of Mammary Pathology, General Surgery Service, University Hospital of Jaén, Jaén, Spain. [Reina-Pérez,I; Fernández,MF] Department of Radiology, School of Medicine, University of Granada, Granada, Spain. [Fernández,MF] Spanish Consortium for Research on Epidemiology and Public Health (CIBERESP), Instituto de Salud Carlos III, Madrid, Spain. [Pérez-Alonso,AJ; Torné,P] Department of Surgery, School of Medicine, University of Granada, Granada, Spain. [Torné,P; Gallart-Aragón,T; Sánchez-Barrón,MT; Reyes Lartategui,S] Unit of Mammary Pathology, General Surgery Service, University Hospital Campus de la Salud, Granada, Spain. [García,F; Chueca,N] Department of Microbiology, University Hospital Campus de la Salud, Granada, Spain. [Moreno-Delgado,A; Torres-Martínez,K] Clínica Ana Moreno, Calle Doctor Alejandro Otero, Granada, Spain. [Sáez-Lara,MJ] Department of Biochemistry and Molecular Biology I, School of Sciences, University of Granada, Granada, Spain. [Fernández,MF] Laboratory 207, Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, Granada, Spain.
Keywords: Archaea;Bacteria;Breast cancer;Breast microbiota;Endocrine disruptors;Environmental pollutants;Fungi;Gut microbiota;Virus;Breast neoplasms;Neoplasias de la mama;Gastrointestinal microbiome;Microbioma sastrointestinal;Disruptores endocrinos;Contaminantes ambientales;Hongos
metadata.dc.subject.mesh: Medical Subject Headings::Persons::Persons::Age Groups::Adult
Medical Subject Headings::Persons::Persons::Age Groups::Adult::Aged
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Biopsy
Medical Subject Headings::Anatomy::Body Regions::Breast
Medical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Breast Neoplasms
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Damage
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Dysbiosis
Medical Subject Headings::Health Care::Environment and Public Health::Public Health::Environmental Pollution::Environmental Exposure
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Estrogens
Medical Subject Headings::Anatomy::Fluids and Secretions::Feces
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolome
Medical Subject Headings::Persons::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Endocrine Disruptors
Medical Subject Headings::Organisms::Eukaryota::Fungi
Medical Subject Headings::Organisms::Viruses
Medical Subject Headings::Organisms::Archaea
Issue Date: 24-May-2019
Citation: Plaza-Díaz J, Álvarez-Mercado AI, Ruiz-Marín CM, Reina-Pérez I, Pérez-Alonso AJ, Sánchez-Andujar MB, et al. Association of breast and gut microbiota dysbiosis and the risk of breast cancer: a case-control clinical study. BMC Cancer. 2019 May 24;19(1):495.
Abstract: Background: Breast cancer ranks first in women, and is the second cause of death in this gender. In addition to genetics, the environment contributes to the development of the disease, although the factors involved are not well known. Among the latter is the influence of microorganisms and, therefore, attention is recently being paid to the mammary microbiota. We hypothesize that the risk of breast cancer could be associated with the composition and functionality of the mammary/gut microbiota, and that exposure to environmental contaminants (endocrine disruptors, EDCs) might contribute to alter these microbiota. Methods: We describe a case-control clinical study that will be performed in women between 25 and 70 years of age. Cases will be women diagnosed and surgically intervened of breast cancer (stages I and II). Women with antecedents of cancer or advanced tumor stage (metastasis), or who have received antibiotic treatment within a period of 3 months prior to recruitment, or any neoadjuvant therapy, will be excluded. Controls will be women surgically intervened of breast augmentation or reduction. Women with oncological, gynecological or endocrine history, and those who have received antibiotic treatment within a period of 3 months prior to recruitment will also be excluded. Blood, urine, breast tissue and stool samples will be collected. Data regarding anthropometric, sociodemographic, reproductive history, tumor features and dietary habits will be gathered. Metabolomic studies will be carried out in stool and breast tissue samples. Metagenomic studies will also be performed in stool and breast tissue samples to ascertain the viral, fungal, bacterial and archaea populations of the microbiota. Quantitation of estrogens, estrogen metabolites and EDCs in samples of serum, urine and breast tissue will also be performed. Discussion: This is the first time that the contribution of bacteria, archaea, viruses and fungi together with their alteration by environmental contaminants to the risk of breast cancer will be evaluated in the same study. Results obtained could contribute to elucidate risk factors, improve the prognosis, as well as to propose novel intervention studies in this disease.
URI: http://hdl.handle.net/10668/3162
metadata.dc.relation.publisherversion: https://bmccancer.biomedcentral.com/articles/10.1186/s12885-019-5660-y
metadata.dc.identifier.doi: 10.1186/s12885-019-5660-y
ISSN: 1471-2407 (Online)
Appears in Collections:01- Artículos - Complejo Hospitalario de Jaén
01- Artículos - Complejo Hospitalario Universitario de Granada
01- Artículos - ibsGRANADA. Instituto de Investigación Biosanitaria de Granada

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