Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/3165
Title: Crosstalk between chromatin structure, cohesin activity and transcription
Authors: Maya-Miles, Douglas
Andújar, Eloísa
Pérez-Alegre, Mónica
Murillo-Pineda, Marina
Barrientos-Moreno, Marta
Cabello-Lobato, María J.
Gómez-Marín, Elena
Morillo-Huesca, Macarena
Prado, Félix
metadata.dc.contributor.authoraffiliation: [Maya-Miles,D; Murillo-Pineda,M; Barrientos-Moreno,M; Cabello-Lobato,MJ; Gomez-Marin,E; Morillo-Huesca,M; Prado,F] Department of Genome Biology, Andalusian Molecular Biology and Regenerative Medicine (CABIMER), CSIC-University of Seville-University Pablo de Olavide, Seville, Spain. [Andujar,E; Perez-Alegre,M] Genomic Unit, Andalusian Molecular Biology and Regenerative Medicine Center (CABIMER), CSIC-University of Seville-University Pablo de Olavide, Seville, Spain. [Murillo-Pineda,M] Department of Biochemistry, University of Oxford, Oxford, UK. [Cabello-Lobato,MJ] Division of Cancer Sciences, Manchester Cancer Research Center, University of Manchester, Manchester, UK.
Keywords: Chromatin;Cohesin;Transcription;Scc1;Cromatina;Transcripción genética
metadata.dc.subject.mesh: Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Cell Cycle Proteins
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cell Nucleus::Cell Nucleus Structures::Intranuclear Space::Chromosomes::Chromosome Structures::Chromatin
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Packaging::Chromatin Assembly and Disassembly
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nuclear Proteins::Chromosomal Proteins, Non-Histone
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Replication
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Down-Regulation
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Genetic Association Studies::Genome-Wide Association Study
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nuclear Proteins::Histones
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cell Nucleus::Cell Nucleus Structures::Intranuclear Space::Chromosomes::Chromosome Structures::Chromatin::Nucleosomes
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Gene Components::Regulatory Elements, Transcriptional::Promoter Regions, Genetic
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Protein Binding
Medical Subject Headings::Organisms::Eukaryota::Fungi::Ascomycota::Saccharomycetales::Saccharomyces::Saccharomyces cerevisiae
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression::Transcription, Genetic
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Transcriptional Activation
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Up-Regulation
Issue Date: 22-Jul-2019
Publisher: BioMed Central Ltd
Citation: Maya-Miles D, Andújar E, Pérez-Alegre M, Murillo-Pineda M, Barrientos-Moreno M, Cabello-Lobato MJ, et al. Crosstalk between chromatin structure, cohesin activity and transcription. Epigenetics Chromatin. 2019 Jul 22;12(1):47.
Abstract: Background: A complex interplay between chromatin and topological machineries is critical for genome architecture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its multiple roles in DNA metabolism. Results: We have used genome-wide analyses to address how cohesins and chromatin structure impact each other in yeast. Cohesin inactivation in scc1-73 mutants during the S and G2 phases causes specific changes in chromatin structure that preferentially take place at promoters; these changes include a significant increase in the occupancy of the - 1 and + 1 nucleosomes. In addition, cohesins play a major role in transcription regulation that is associated with specific promoter chromatin architecture. In scc1-73 cells, downregulated genes are enriched in promoters with short or no nucleosome-free region (NFR) and a fragile "nucleosome - 1/RSC complex" particle. These results, together with a preferential increase in the occupancy of nucleosome - 1 of these genes, suggest that cohesins promote transcription activation by helping RSC to form the NFR. In sharp contrast, the scc1-73 upregulated genes are enriched in promoters with an "open" chromatin structure and are mostly at cohesin-enriched regions, suggesting that a local accumulation of cohesins might help to inhibit transcription. On the other hand, a dramatic loss of chromatin integrity by histone depletion during DNA replication has a moderate effect on the accumulation and distribution of cohesin peaks along the genome. Conclusions: Our analyses of the interplay between chromatin integrity and cohesin activity suggest that cohesins play a major role in transcription regulation, which is associated with specific chromatin architecture and cohesin-mediated nucleosome alterations of the regulated promoters. In contrast, chromatin integrity plays only a minor role in the binding and distribution of cohesins.
URI: http://hdl.handle.net/10668/3165
metadata.dc.identifier.doi: 10.1186/s13072-019-0293-6
ISSN: 1756-8935 (Online)
Appears in Collections:01- Artículos - CABIMER. Centro Andaluz de Biología Molecular

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