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Title: Dendrimeric Antigens for Drug Allergy Diagnosis: A New Approach for Basophil Activation Tests
Authors: Molina, Noemi
Martin-Serrano, Angela
Fernandez, Tahia D.
Tesfaye, Amene
Najera, Francisco
Torres, María J.
Mayorga, Cristobalina
Vida, Yolanda
Montañez, Maria I.
Perez-Inestrosa, Ezequiel
metadata.dc.contributor.authoraffiliation: [Molina,N; Najera,F; Vida,Y; Perez-Inestrosa,E] Departamento de Química Orgánica, Universidad de Málaga—IBIMA, Málaga, Spain. [Molina,N; Martin-Serrano,A; Fernandez,TD, Tesfaye,A; Najera,F; Torres,MJ; Mayorga,C; Vida,Y; Montañez,MI; Perez-Inestrosa,E] Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Parque Tecnológico de Andalucía, Málaga, Spain. [Martin-Serrano,A; Fernandez,TD; Tesfaye,A; Mayorga,C; Montañez,MI] Research Laboratory, IBIMA—Regional University Hospital of Málaga—UMA, Málaga, Spain. [Torres,MJ; Mayorga,C] Allergy Unit, IBIMA—Regional University Hospital of Málaga—UMA,Málaga, Spain
Keywords: PAMAM;Dendrimeric antigens;Penicillin;Drug allergy;Basophil activation tests;Penicilinas;Hipersensibilidad a las drogas;Prueba de desgranulación de los Basófilos
metadata.dc.subject.mesh: Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergens
Medical Subject Headings::Anatomy::Cells::Myeloid Cells::Granulocytes::Basophils
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Dendrimers
Medical Subject Headings::Diseases::Chemically-Induced Disorders::Drug-Related Side Effects and Adverse Reactions::Drug Hypersensitivity
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Epitopes
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Spectrum Analysis::Magnetic Resonance Spectroscopy
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Theoretical::Models, Molecular
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Penicillins
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Structure-Activity Relationship
Issue Date: 24-Apr-2018
Publisher: MDPI
Citation: Molina N, Martin-Serrano A, Fernandez TD, Tesfaye A, Najera F, Torres MJ, et al. Dendrimeric Antigens for Drug Allergy Diagnosis: A New Approach for Basophil Activation Tests. Molecules. 2018 Apr 24;23(5):997.
Abstract: Dendrimeric Antigens (DeAns) consist of dendrimers decorated with multiple units of drug antigenic determinants. These conjugates have been shown to be a powerful tool for diagnosing penicillin allergy using in vitro immunoassays, in which they are recognized by specific IgE from allergic patients. Here we propose a new diagnostic approach using DeAns in cellular tests, in which recognition occurs through IgE bound to the basophil surface. Both IgE molecular recognition and subsequent cell activation may be influenced by the tridimensional architecture and size of the immunogens. Structural features of benzylpenicilloyl-DeAn and amoxicilloyl-DeAn (G2 and G4 PAMAM) were studied by diffusion Nuclear Magnetic Resonance (NMR) experiments and are discussed in relation to molecular dynamics simulation (MDS) observations. IgE recognition was clinically evaluated using the basophil activation test (BAT) for allergic patients and tolerant subjects. Diffusion NMR experiments, MDS and cellular studies provide evidence that the size of the DeAn, its antigen composition and tridimensional distribution play key roles in IgE-antigen recognition at the effector cell surface. These results indicate that the fourth generation DeAns induce a higher level of basophil activation in allergic patients. This approach can be considered as a potential complementary diagnostic method for evaluating penicillin allergy.
metadata.dc.identifier.doi: 10.3390/molecules23050997
ISSN: 1420-3049 (Online)
Appears in Collections:01- Artículos - BIONAND - Centro Andaluz de Nanomedicina y Biotecnología
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

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