Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/335
Title: The Thioredoxin TRX-1 Modulates the Function of the Insulin-Like Neuropeptide DAF-28 during Dauer Formation in Caenorhabditis elegans
Authors: Fierro-González, Juan Carlos.
Cornils, Astrid.
Alcedo, Joy
Miranda-Vizuete, Antonio.
Swoboda, Peter.
metadata.dc.contributor.authoraffiliation: [Fierro-González,JC; Swoboda,P] Department of Biosciences and Nutrition, Center for Biosciences at NOVUM, Karolinska Institute, Huddinge, Sweden. [Cornils,A; Alcedo,J] Friedrich Miescher Institute for Biomedical Research, Switzerland. [Miranda-Vizuete,A] Departamento de Fisiología, Anatomía y Biología Celular, Centro Andaluz de Biología del Desarrollo - Consejo Superior de Investigaciones Científicas (CABD-CSIC), Universidad Pablo de Olavide, Sevilla, Spain. Instituto de Biomedicina de Sevilla. Hospital Universitario Virgen del Rocío. Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, Sevilla, Spain.
Keywords: Tiorredoxinas;Proteínas de Caenorhabditis elegans
metadata.dc.subject.mesh: Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Thioredoxins
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Helminth Proteins::Caenorhabditis elegans Proteins
Issue Date: 27-Jan-2011
Publisher: PLoS
Citation: Fierro-González JC, Cornils A, Alcedo J, Miranda-Vizuete A, Swoboda P. The Thioredoxin TRX-1 Modulates the Function of the Insulin-Like Neuropeptide DAF-28 during Dauer Formation in Caenorhabditis elegans. PLoS ONE 2011; 6(1): e16561.
Abstract: Thioredoxins comprise a conserved family of redox regulators involved in many biological processes, including stress resistance and aging. We report that the C. elegans thioredoxin TRX-1 acts in ASJ head sensory neurons as a novel modulator of the insulin-like neuropeptide DAF-28 during dauer formation. We show that increased formation of stress-resistant, long-lived dauer larvae in mutants for the gene encoding the insulin-like neuropeptide DAF-28 requires TRX-1 acting in ASJ neurons, upstream of the insulin-like receptor DAF-2. Genetic rescue experiments demonstrate that redox-independent functions of TRX-1 specifically in ASJ neurons are needed for the dauer formation constitutive (Daf-c) phenotype of daf-28 mutants. GFP reporters of trx-1 and daf-28 show opposing expression patterns in dauers (i.e. trx-1 is up-regulated and daf-28 is down-regulated), an effect that is not observed in growing L2/L3 larvae. In addition, functional TRX-1 is required for the down-regulation of a GFP reporter of daf-28 during dauer formation, a process that is likely subject to DAF-28-mediated feedback regulation. Our findings demonstrate that TRX-1 modulates DAF-28 signaling by contributing to the down-regulation of daf-28 expression during dauer formation. We propose that TRX-1 acts as a fluctuating neuronal signaling modulator within ASJ neurons to monitor the adjustment of neuropeptide expression, including insulin-like proteins, during dauer formation in response to adverse environmental conditions.
URI: http://hdl.handle.net/10668/335
metadata.dc.relation.publisherversion: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0016561
metadata.dc.identifier.doi: 10.1371/journal.pone.0016561
ISSN: 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Virgen del Rocío
01- Artículos - IBIS. Instituto de Biomedicina de Sevilla

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