Por favor, usa este identificador para citar o enlazar este ítem: http://hdl.handle.net/10668/3462
Título : The Effect of Pore Directionality of Collagen Scaffolds on Cell Differentiation and In Vivo Osteogenesis
Autor : Moncayo-Donoso, Miguelangel
Rico-Llanos, Gustavo A.
Garzón-Alvarado, Diego A.
Becerra, José
Visser, Rick
Fontanilla, Marta R.
Filiación: [Moncayo-Donoso,M; Fontanilla,MR] Tissue Engineering Group, Department of Pharmacy, Universidad Nacional de Colombia, Bogotá, Colombia. [Moncayo-Donoso,M; Fontanilla,MR] [Moncayo-Donoso,M; Garzón-Alvarado,DA] Biomimetics Laboratory, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia. [Moncayo-Donoso,M; Rico-Llanos,GA; Becerra,J; Visser,R] BIONAND, Andalusian Center for Nanomedicine and Biotechnology, University of Malaga, Malaga, Spain. [Rico-Llanos,GA; Becerra,J; Visser,R] Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, Malaga, Spain. [Becerra,J] Department of Cell Biology, Genetics and Physiology, University of Malaga, IBIMA, Malaga, Spain.
Palabras clave : BMP-2;VEGF;Bone tissue engineering;Osteogenesis;Pore directionality;Type I collagen;Proteína morfogenética ósea 2;Osteogénesis;Colágeno Tipo I
MeSH: Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Morphogenesis::Embryonic and Fetal Development::Organogenesis::Musculoskeletal Development::Bone Development::Osteogenesis
Medical Subject Headings::Chemicals and Drugs::Biomedical and Dental Materials::Biocompatible Materials::Bone Substitutes
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Porosity
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Angiogenic Proteins::Vascular Endothelial Growth Factors::Vascular Endothelial Growth Factor A
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Tensile Strength
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen::Fibrillar Collagens::Collagen Type I
Fecha de publicación : 20-sep-2021
Editorial : MDPI AG
Cita Bibliográfica: Moncayo-Donoso M, Rico-Llanos GA, Garzón-Alvarado DA, Becerra J, Visser R, Fontanilla MR. The Effect of Pore Directionality of Collagen Scaffolds on Cell Differentiation and In Vivo Osteogenesis. Polymers (Basel). 2021 Sep 20;13(18):3187.
Abstract: Although many bone substitutes have been designed and produced, the development of bone tissue engineering products that mimic the microstructural characteristics of native bone remains challenging. It has been shown that pore orientation within collagen scaffolds influences bone matrix formation by the endochondral route. In addition, that the unidirectional orientation of the scaffolds can limit the growth of blood vessels. However, a comparison between the amount of bone that can be formed in scaffolds with different pore orientations in addition to analyzing the effect of loading osteogenic and proangiogenic factors is still required. In this work we fabricated uni- and multidirectional collagen sponges and evaluated their microstructural, physicochemical, mechanical and biological characteristics. Although the porosity and average pore size of the uni- and multidirectional scaffolds was similar (94.5% vs. 97.1% and 260 µm vs. 269 µm, respectively) the unidirectional sponges had a higher tensile strength, Young's modulus and capacity to uptake liquids than the multidirectional ones (0.271 MPa vs. 0.478 MPa, 9.623 MPa vs. 3.426 MPa and 8000% mass gain vs. 4000%, respectively). Culturing of rat bone marrow mesenchymal stem cells demonstrated that these scaffolds support cell growth and osteoblastic differentiation in the presence of BMP-2 in vitro, although the pore orientation somehow affected cell attachment and differentiation. The evaluation of the ability of the scaffolds to support bone growth when loaded with BMP-2 or BMP-2 + VEGF in an ectopic rat model showed that they both supported bone formation. Histological analysis and quantification of mineralized matrix revealed that the pore orientation of the collagen scaffolds influenced the osteogenic process.
URI: http://hdl.handle.net/10668/3462
Versión del editor : https://www.mdpi.com/2073-4360/13/18/3187
DOI: 10.3390/polym13183187
ISSN : 2073-4360 (Online)
Aparece en las colecciones: 01- Artículos - BIONAND - Centro Andaluz de Nanomedicina y Biotecnología
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
MoncayoDonoso_EffectOfPore.pdfArtículo original6,99 MBAdobe PDFVisualizar/Abrir

Este ítem está protegido por copyright original

Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons