Please use this identifier to cite or link to this item:
Title: Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming
Authors: Lopez-Caraballo, Lidia
Martorell-Marugan, Jordi
Carmona-Saez, Pedro
Gonzalez-Muñoz, Elena
metadata.dc.contributor.authoraffiliation: [Lopez-Caraballo,L; Gonzalez-Muñoz,E] Andalusian Laboratory of Cell Reprogramming (LARCel), Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain. [Martorell-Marugan,J; Carmona-Saez,P] Bioinformatics Unit. GENYO, Centre for Genomics and Oncological Research: Pfizer/University of Granada/Andalusian Regional Government, Granada, Spain. [Gonzalez-Muñoz,E] Department of Cell Biology, Genetics and Physiology, University of Malaga, Málaga, Spain. [Gonzalez-Muñoz,E] Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Málaga, Spain. [Martorell-Marugan,J] Atrys Health, Barcelona, Spain. [Carmona-Saez,P] Department of Statistics. University of Granada, Spain.
Keywords: Stromal cells;Menstruation;Blood cells;Cellular reprogramming;Oocytes;Células del estroma;Menstruación;Células sanguíneas;Reprogramación celular;Oocitos
metadata.dc.subject.mesh: Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::Metaphase
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Molecular Chaperones::Histone Chaperones
Medical Subject Headings::Anatomy::Cells::Germ Cells::Ovum::Oocytes
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells
Medical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Menstrual Cycle::Menstruation
Issue Date: 21-Aug-2020
Publisher: Cell Press, Elsevier
Citation: Lopez-Caraballo L, Martorell-Marugan J, Carmona-Saez P, Gonzalez-Muñoz E. Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming. iScience. 2020 Aug 21;23(8):101376
Abstract: Cell reprogramming has revolutionized cell and regenerative biology field. However, human iPS derivation remains inefficient and variable. A better knowledge of molecular processes and the rationale underlying the importance of somatic cell origin is crucial to uncover reprogramming mechanisms. Here, we analyze the molecular profile of different human somatic cell types. We show menstrual blood-derived stromal cells (MnSCs) have a distinct, reprogramming prone, profile, and we identify SOX15 from their oocyte-related signature as a prominent responsible candidate. SOX15 orchestrates an efficient oocyte-based reprogramming combination when overexpressed with the also oocyte-enriched histone chaperone ASF1A and OCT4 and, through specific mechanism, generates iPSCs with distinguishable pluripotent state that further present higher differentiation capacity than canonical iPSCs. Our work supports the presence of different pluripotency states in reprogramming and the importance of using metaphase-II oocyte and MnSCs information to provide alternative reprogramming combinations and, importantly, to improve and understand pluripotency acquisition.
metadata.dc.identifier.doi: 10.1016/j.isci.2020.101376
ISSN: 2589-0042 (Online)
Appears in Collections:01- Artículos - BIONAND - Centro Andaluz de Nanomedicina y Biotecnología
01- Artículos - GENYO. Centro Pfizer-Andalucía de Genómica e Investigación Oncológica

Files in This Item:
File Description SizeFormat 
LopezCaraballo_AnalysisOfMenstrual.pdfArtículo publicado7,01 MBAdobe PDFView/Open
LopezCaraballo_AnalysisOfMenstrual_Figuras.pdfMaterial suplementario2,44 MBAdobe PDFView/Open
LopezCaraballo_AnalysisOfMenstrual_Tabla1.xlsxMaterial suplementario1,49 MBMicrosoft Excel XMLView/Open
LopezCaraballo_AnalysisOfMenstrual_Tabla2.xlsxMaterial suplementario13,59 MBMicrosoft Excel XMLView/Open
LopezCaraballo_AnalysisOfMenstrual_Tabla3.xlsxMaterial suplementario8,09 MBMicrosoft Excel XMLView/Open
LopezCaraballo_AnalysisOfMenstrual_Tabla4.xlsxMaterial suplementario11,44 kBMicrosoft Excel XMLView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons