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Title: Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites
Authors: Rodriguez-Gallardo, Sofia
Kurokawa, Kazuo
Sabido-Bozo, Susana
Cortes-Gomez, Alejandro
Ikeda, Atsuko
Zoni, Valeria
Aguilera-Romero, Auxiliadora
Perez-Linero, Ana Maria
Lopez, Sergio
Waga, Miho
Araki, Misako
Nakano, Miyako
Riezman, Howard
Funato, Kouichi
Vanni, Stefano
Nakano, Akihiko
Muñiz, Manuel
metadata.dc.contributor.authoraffiliation: [Rodriguez-Gallardo,S; Sabido-Bozo,S; Cortes-Gomez,A; Aguilera-Romero,A; Perez-Linero,AM; Lopez,S; Muñiz,M] Department of Cell Biology, Faculty of Biology, University of Seville and Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain. [Kurokawa,K; Waga,M; Nakano,A] Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Saitama, Japan. [Zoni,V; Vanni;S] Department of Biology, University of Fribourg, Fribourg, Switzerland. [Ikeda,A; Araki,M; Nakano,M; Funato,K] Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan. [Riezman,H] NCCR Chemical Biology, Department of Biochemistry, University of Geneva, Geneva, Switzerland.
Keywords: Ceramides;Glycosylphosphatidylinositol;Endoplasmic reticulum;Lipids;Membranes;Proteins;Reticulum;Secretory pathway;Ceramidas;Retículo endoplásmico;Lípidos;Proteínas;Reticulum;Vías secretorias;Membranas;Glicosilfosfatidilinositoles
metadata.dc.subject.mesh: Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Biological Transport::Protein Transport
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Ceramides
Medical Subject Headings::Chemicals and Drugs::Lipids::Glycolipids::Glycosylphosphatidylinositols
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Endoplasmic Reticulum
Medical Subject Headings::Chemicals and Drugs::Lipids
Medical Subject Headings::Anatomy::Tissues::Membranes
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins
Medical Subject Headings::Anatomy::Animal Structures::Stomach, Ruminant::Reticulum
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Biological Transport::Secretory Pathway
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Homeostasis
Issue Date: 11-Dec-2020
Publisher: American Association for the Advancement of Science.
Citation: Rodriguez-Gallardo S, Kurokawa K, Sabido-Bozo S, Cortes-Gomez A, Ikeda A, Zoni V, et al. Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites. Sci Adv. 2020 Dec 11;6(50):eaba8237
Abstract: Protein sorting in the secretory pathway is crucial to maintain cellular compartmentalization and homeostasis. In addition to coat-mediated sorting, the role of lipids in driving protein sorting during secretory transport is a longstanding fundamental question that still remains unanswered. Here, we conduct 3D simultaneous multicolor high-resolution live imaging to demonstrate in vivo that newly synthesized glycosylphosphatidylinositol-anchored proteins having a very long chain ceramide lipid moiety are clustered and sorted into specialized endoplasmic reticulum exit sites that are distinct from those used by transmembrane proteins. Furthermore, we show that the chain length of ceramide in the endoplasmic reticulum membrane is critical for this sorting selectivity. Our study provides the first direct in vivo evidence for lipid chain length-based protein cargo sorting into selective export sites of the secretory pathway.
metadata.dc.identifier.doi: 10.1126/sciadv.aba8237
ISSN: 2375-2548 (Online)
Appears in Collections:01- Artículos - Hospital Virgen del Rocío
01- Artículos - IBIS. Instituto de Biomedicina de Sevilla

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