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Title: Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas
Authors: Garzón, Ingrid
Chato-Astrain, Jesus
González-Gallardo, Carmen
Ionescu, Ana
Cardona, Juan de la Cruz
Mateu, Miguel
Carda, Carmen
Pérez, María del Mar
Martín-Piedra, Miguel Ángel
Alaminos, Miguel
metadata.dc.contributor.authoraffiliation: [Garzón,I; Chato-Astrain,J; Mateu,M; Martín-Piedra,MM; Alaminos,M] Tissue Engineering Group, Department of Histology, University of Granada, Granada, Spain. [Garzón,I; Chato-Astrain,J; Mateu,M; Martín-Piedra,MM; Alaminos,M] Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain. [González-Gallardo,C] Division of ophthalmology, University Hospital San Cecilio, Granada, Spain. [Ionescu,A; Cardona,JC; Pérez,MM] Biomaterials Optics Group, Department of Optics, University of Granada, Granada, Spain. [Carda,C] Department of Histology and Pathology, University of Valencia, Valencia, Spain.
Keywords: Tissue engineering;Bioengineered cornea;Wharton’s jelly stem cells;Heterotypical human cornea;Artificial cornea;Ingeniería de tejidos;Células madre mesenquimatosas;Córnea
metadata.dc.subject.mesh: Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Engineering::Bioengineering::Cell Engineering::Tissue Engineering
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells
Medical Subject Headings::Anatomy::Sense Organs::Eye::Anterior Eye Segment::Cornea
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Wharton Jelly
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Optical Imaging::Tomography, Optical::Tomography, Optical Coherence
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Fibrin
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharose
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Lagomorpha::Rabbits
Medical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Biocompatible Materials
Medical Subject Headings::Anatomy::Cells::Epithelial Cells
Medical Subject Headings::Anatomy::Embryonic Structures::Fetus::Umbilical Cord
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal
Issue Date: 19-Jun-2020
Publisher: Frontiers
Citation: Garzón I, Chato-Astrain J, González-Gallardo C, Ionescu A, Cardona JC, Mateu M, et al. Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas. Front Bioeng Biotechnol. 2020 Jun 19;8:681.
Abstract: Purpose: Human cornea substitutes generated by tissue engineering currently require limbal stem cells for the generation of orthotypical epithelial cell cultures. We recently reported that bioengineered corneas can be fabricated in vitro from a heterotypical source obtained from Wharton’s jelly in the human umbilical cord (HWJSC). Methods: Here, we generated a partial thickness cornea model based on plastic compression nanostructured fibrin-agarose biomaterials with cornea epithelial cells on top, as an orthotypical model (HOC), or with HWJSC, as a heterotypical model (HHC), and determined their potential in vivo usefulness by implantation in an animal model. Results: No major side effects were seen 3 and 12 months after implantation of either bioengineered partial cornea model in rabbit corneas. Clinical results determined by slit lamp and optical coherence tomography were positive after 12 months. Histological and immunohistochemical findings demonstrated that in vitro HOC and HHC had moderate levels of stromal and epithelial cell marker expression, whereas in vivo grafted corneas were more similar to control corneas. Conclusion: These results suggest that both models are potentially useful to treat diseases requiring anterior cornea replacement, and that HHC may be an efficient alternative to the use of HOC which circumvents the need to generate cornea epithelial cell cultures.
metadata.dc.identifier.doi: 10.3389/fbioe.2020.00681
ISSN: 2296-4185 (Online)
Appears in Collections:01- Artículos - Hospital San Cecilio
01- Artículos - ibsGRANADA. Instituto de Investigación Biosanitaria de Granada

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