Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/372
Título : Prevalence and resistance mutations of non-B HIV-1 subtypes among immigrants in Southern Spain along the decade 2000-2010.
Autor : de Felipe, Beatriz
Pérez-Romero, Pilar
Abad-Fernández, María.
Fernandez-Cuenca, Felipe
Martinez-Fernandez, Francisco J.
Trastoy, Mónica
Mata, Rosario C
López-Cortés, Luis F.
Leal, Manuel
Viciana, Pompeyo
Vallejo, Alejandro
Filiación: [de Felipe,B; Pérez-Romero,P; Martinez-Fernandez,FJ; Trastoy,M; Mata,RC; López-Cortés,LF; Viciana,P] Infectious Diseases Service, IBIS, Hospital Univeritario Virgen del Rocio, Seville, Spain. [Abad-Fernández,M ; Vallejo,A] Laboratory of Immunovirology, Infectious Diseases Service, Instituto Ramón y Cajal de Investigaciones Cientificas (IRYCIS), Hospital Universitario Ramón y Cajal, Madrid, Spain.[Fernandez-Cuenca,F] Microbiology Service Hospital Virgen Macarena Seville, Spain. [Leal,M; Vallejo,A] Laboratory of Immunovirology, Infectious Diseases Service, IBIS, Virgen del Rocío University Hospital, Seville, Spain.
Palabras clave : non-B HIV-1 subtypes
Emigrantes e Inmigrantes
Mutación Missense
Andalucía
MeSH: Medical Subject Headings::Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus::Lentiviruses, Primate::HIV::HIV-1
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Mutation::Mutation, Missense
Medical Subject Headings::Named Groups::Persons::Emigrants and Immigrants
Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Cluster Analysis
Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Drug Resistance, Microbial::Drug Resistance, Viral
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Information Science::Information Science::Data Collection::Vital Statistics::Morbidity::Prevalence
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA
Fecha de publicación : 26-Aug-2011
Editorial : BioMed Central
Cita Bibliográfica: de Felipe B, Pérez-Romero P, Abad-Fernández M, Fernandez-Cuenca F, Martinez-Fernandez FJ,Trastoy M, et al. Prevalence and resistance mutations of non-B HIV-1 subtypes among immigrants in Southern Spain along the decade 2000-2010. Virol J. 2011 Aug 26;8:416.
Abstract: Most of the non-B HIV-1 subtypes are predominant in Sub-Saharan Africa and India although they have been found worldwide. In the last decade, immigration from these areas has increased considerably in Spain. The objective of this study was to evaluate the prevalence of non-B subtypes circulating in a cohort of HIV-1-infected immigrants in Seville, Southern Spain and to identify drug resistance-associated mutations. METHODS: Complete protease and first 220 codons of the reverse transcriptase coding regions were amplified and sequenced by population sequencing. HIV-1 subtypes were determined using Stanford University Drug Resistance Database, and phylogenetic analysis was performed comparing multiple reported sequences. Drug resistance mutations were defined according to the International AIDS Society-USA. RESULTS: From 2000 to 2010 a total of 1,089 newly diagnosed HIV-1-infected patients were enrolled in our cohort. Of these, 121 were immigrants, of which 98 had ethical approval and informed consent to include in our study. Twenty-nine immigrants (29/98, 29.6%) were infected with non-B subtypes, of which 15/29 (51.7%) were CRF02-AG, mostly from Sub-Saharan Africa, and 2/29 (6.9%) were CRF01-AE from Eastern Europe. A, C, F, J and G subtypes from Eastern Europe, Central-South America and Sub-Saharan Africa were also present. Some others harboured recombinant forms CRF02-AG/CRF01-AE, CRF2-AG/G and F/B, B/C, and K/G, in PR and RT-coding regions. Patients infected with non-B subtypes showed a high frequency of minor protease inhibitor resistance mutations, M36I, L63P, and K20R/I. Only one patient, CRF02_AG, showed major resistance mutation L90M. Major RT inhibitor resistance mutations K70R and A98G were present in one patient with subtype G, L100I in one patient with CRF01_AE, and K103N in another patient with CRF01_AE. Three patients had other mutations such as V118I, E138A and V90I. CONCLUSIONS: The circulation of non-B subtypes has significantly increased in Southern Spain during the last decade, with 29.6% prevalence, in association with demographic changes among immigrants. This could be an issue in the treatment and management of these patients. Resistance mutations have been detected in these patients with a prevalence of 7% among treatment-naïve patients compared with the 21% detected among patients under HAART or during treatment interruption.
URI: http://hdl.handle.net/10668/372
Versión del editor : http://www.virologyj.com/content/8/1/416/abstract
DOI: 10.1186/1743-422X-8-416
ISSN : 1743-422X (Online)
Appears in Collections:01- Artículos - Hospital Virgen Macarena
01- Artículos - IBIS. Instituto de Biomedicina de Sevilla
01- Artículos - Hospital Virgen del Rocío

Files in This Item:
File Description SizeFormat 
deFelipe_Prevalence.pdfArtículo publicado893,48 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons