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dc.contributor.authorde Felipe, Beatriz-
dc.contributor.authorPérez-Romero, Pilar-
dc.contributor.authorAbad-Fernández, María.-
dc.contributor.authorFernandez-Cuenca, Felipe-
dc.contributor.authorMartinez-Fernandez, Francisco J.-
dc.contributor.authorTrastoy, Mónica-
dc.contributor.authorMata, Rosario C-
dc.contributor.authorLópez-Cortés, Luis F.-
dc.contributor.authorLeal, Manuel-
dc.contributor.authorViciana, Pompeyo-
dc.contributor.authorVallejo, Alejandro-
dc.identifier.citationde Felipe B, Pérez-Romero P, Abad-Fernández M, Fernandez-Cuenca F, Martinez-Fernandez FJ,Trastoy M, et al. Prevalence and resistance mutations of non-B HIV-1 subtypes among immigrants in Southern Spain along the decade 2000-2010. Virol J. 2011 Aug 26;
dc.identifier.issn1743-422X (Online)-
dc.description.abstractMost of the non-B HIV-1 subtypes are predominant in Sub-Saharan Africa and India although they have been found worldwide. In the last decade, immigration from these areas has increased considerably in Spain. The objective of this study was to evaluate the prevalence of non-B subtypes circulating in a cohort of HIV-1-infected immigrants in Seville, Southern Spain and to identify drug resistance-associated mutations. METHODS: Complete protease and first 220 codons of the reverse transcriptase coding regions were amplified and sequenced by population sequencing. HIV-1 subtypes were determined using Stanford University Drug Resistance Database, and phylogenetic analysis was performed comparing multiple reported sequences. Drug resistance mutations were defined according to the International AIDS Society-USA. RESULTS: From 2000 to 2010 a total of 1,089 newly diagnosed HIV-1-infected patients were enrolled in our cohort. Of these, 121 were immigrants, of which 98 had ethical approval and informed consent to include in our study. Twenty-nine immigrants (29/98, 29.6%) were infected with non-B subtypes, of which 15/29 (51.7%) were CRF02-AG, mostly from Sub-Saharan Africa, and 2/29 (6.9%) were CRF01-AE from Eastern Europe. A, C, F, J and G subtypes from Eastern Europe, Central-South America and Sub-Saharan Africa were also present. Some others harboured recombinant forms CRF02-AG/CRF01-AE, CRF2-AG/G and F/B, B/C, and K/G, in PR and RT-coding regions. Patients infected with non-B subtypes showed a high frequency of minor protease inhibitor resistance mutations, M36I, L63P, and K20R/I. Only one patient, CRF02_AG, showed major resistance mutation L90M. Major RT inhibitor resistance mutations K70R and A98G were present in one patient with subtype G, L100I in one patient with CRF01_AE, and K103N in another patient with CRF01_AE. Three patients had other mutations such as V118I, E138A and V90I. CONCLUSIONS: The circulation of non-B subtypes has significantly increased in Southern Spain during the last decade, with 29.6% prevalence, in association with demographic changes among immigrants. This could be an issue in the treatment and management of these patients. Resistance mutations have been detected in these patients with a prevalence of 7% among treatment-naïve patients compared with the 21% detected among patients under HAART or during treatment
dc.description.sponsorshipThis study was supported in part by Fondo de Investigación Sanitaria (FIS) 07/0070, Redes Temáticas de Investigación Cooperativa en Salud (RETICS) Red de Investigación en SIDA (RIS) RD06/0006/0021 and RD/06/0006/0034, and Servicio Andaluz de Salud, Consejería de Salud 0407/
dc.publisherBioMed Centrales
dc.relation.ispartofVirology Journales
dc.subjectnon-B HIV-1 subtypeses
dc.subjectEmigrantes e Inmigranteses
dc.subjectMutación Missensees
dc.subject.meshMedical Subject Headings::Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus::Lentiviruses, Primate::HIV::HIV-1es
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Mutation::Mutation, Missensees
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Emigrants and Immigrantses
dc.subject.meshMedical Subject Headings::Geographicals::Geographic Locations::Europe::Spaines
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotypees
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Cluster Analysises
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Drug Resistance, Microbial::Drug Resistance, Virales
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Information Science::Information Science::Data Collection::Vital Statistics::Morbidity::Prevalencees
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNAes
dc.titlePrevalence and resistance mutations of non-B HIV-1 subtypes among immigrants in Southern Spain along the decade
dc.rights.accessRightsAcceso abiertoes
dc.contributor.authoraffiliation[de Felipe,B; Pérez-Romero,P; Martinez-Fernandez,FJ; Trastoy,M; Mata,RC; López-Cortés,LF; Viciana,P] Infectious Diseases Service, IBIS, Hospital Univeritario Virgen del Rocio, Seville, Spain. [Abad-Fernández,M ; Vallejo,A] Laboratory of Immunovirology, Infectious Diseases Service, Instituto Ramón y Cajal de Investigaciones Cientificas (IRYCIS), Hospital Universitario Ramón y Cajal, Madrid, Spain.[Fernandez-Cuenca,F] Microbiology Service Hospital Virgen Macarena Seville, Spain. [Leal,M; Vallejo,A] Laboratory of Immunovirology, Infectious Diseases Service, IBIS, Virgen del Rocío University Hospital, Seville,
Appears in Collections:01- Artículos - Hospital Virgen Macarena
01- Artículos - IBIS. Instituto de Biomedicina de Sevilla
01- Artículos - Hospital Virgen del Rocío

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