Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/376
Title: Mitochondrial dysfunction and mitophagy activation in blood mononuclear cells of fibromyalgia patients: implications in the pathogenesis of the disease.
Authors: Cordero, Mario D.
De Miguel, Manuel
Moreno Fernández, Ana M.
Carmona López, Inés M.
Garrido Maraver, Juan
Cotán, David.
Gómez-Izquierdo, Lourdes
Bonal, Pablo
Campa, Francisco
Bullon, Pedro
Navas, Plácido
Sánchez Alcázar, José A.
metadata.dc.contributor.authoraffiliation: [Cordero,MD; Garrido Maraver,J; Cotán,D; Navas,P; Sánchez Alcázar,JA] Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide-CSIC, ISCIII, Sevilla, Spain. [Cordero,MD; Garrido Maraver,J; Cotán,D; Navas,P; Sánchez Alcázar,JA] Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Universidad Pablo de Olavide-CSIC, ISCIII, Sevilla, Spain. [Cordero,MD; De Miguel,M; Moreno Fernández,AM; Carmona López,IM.] Dpto. Citología e Histología Normal y Patológica, Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain. [Gómez-Izquierdo,L] Departamento de Anatomía Patológica. Hospital Virgen del Rocío, Sevilla, Spain. [Bonal,P; Campa,F] Dpto. de Medicina, Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain. [Campa,F] Distrito Sanitario Sevilla Sur. Facultad de Odontología, Universidad de Sevilla, Sevilla, Spain. [Bullon,P] Departamento de Periodontología, Facultad de Odontología, Universidad de Sevilla, Sevilla, Spain
Keywords: Mitocondrias;Fibromialgia;Leucocitos Mononucleares
metadata.dc.subject.mesh: Medical Subject Headings::Diseases::Musculoskeletal Diseases::Muscular Diseases::Mitochondrial Myopathies
Medical Subject Headings::Diseases::Musculoskeletal Diseases::Muscular Diseases::Fibromyalgia
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative Stress
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Cytophotometry::Flow Cytometry
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Endocytosis::Phagocytosis::Autophagy
Issue Date: 28-Jan-2010
Publisher: BioMed Central
Citation: Cordero MD, De Miguel M, Moreno Fernández AM, Carmona López IM, Garrido Maraver J, Cotán D, et al. Mitochondrial dysfunction and mitophagy activation in blood mononuclear cells of fibromyalgia patients: implications in the pathogenesis of the disease. Arthritis Res Ther. 2010;12(1):R17
Abstract: Introduction. Fibromyalgia is a chronic pain syndrome with unknown etiology. Recent studies have shown some evidence demonstrating that oxidative stress may have a role in the pathophysiology of fibromyalgia. However, it is still not clear whether oxidative stress is the cause or the effect of the abnormalities documented in fibromyalgia. Furthermore, the role of mitochondria in the redox imbalance reported in fibromyalgia also is controversial. We undertook this study to investigate the role of mitochondrial dysfunction, oxidative stress, and mitophagy in fibromyalgia. Methods. We studied 20 patients (2 male, 18 female patients) from the database of the Sevillian Fibromyalgia Association and 10 healthy controls. We evaluated mitochondrial function in blood mononuclear cells from fibromyalgia patients measuring, coenzyme Q10 levels with high-performance liquid chromatography (HPLC), and mitochondrial membrane potential with flow cytometry. Oxidative stress was determined by measuring mitochondrial superoxide production with MitoSOX™ and lipid peroxidation in blood mononuclear cells and plasma from fibromyalgia patients. Autophagy activation was evaluated by quantifying the fluorescence intensity of LysoTracker™ Red staining of blood mononuclear cells. Mitophagy was confirmed by measuring citrate synthase activity and electron microscopy examination of blood mononuclear cells. Results. We found reduced levels of coenzyme Q10, decreased mitochondrial membrane potential, increased levels of mitochondrial superoxide in blood mononuclear cells, and increased levels of lipid peroxidation in both blood mononuclear cells and plasma from fibromyalgia patients. Mitochondrial dysfunction was also associated with increased expression of autophagic genes and the elimination of dysfunctional mitochondria with mitophagy. Conclusions. These findings may support the role of oxidative stress and mitophagy in the pathophysiology of fibromyalgia.
URI: http://hdl.handle.net/10668/376
metadata.dc.relation.publisherversion: http://arthritis-research.com/content/12/1/R17
metadata.dc.identifier.doi: 10.1186/ar2918
ISSN: 1478-6362 (Online)
Appears in Collections:01- Artículos - DSAP Sevilla Sur
01- Artículos - Hospital Virgen del Rocío

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