Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/422
Título : Polymorphisms of CD16A and CD32 Fcgamma receptors and circulating immune complexes in Meniere's disease: a case-control study.
Autor : Lopez-Escamez, Jose A
Saenz-Lopez, Pablo
Gazquez, Irene
Moreno, Antonia
Gonzalez-Oller, Carlos
Soto-Varela, Andrés
Santos, Sofía
Aran, Ismael
Perez-Garrigues, Herminio
Ibañez, Águeda
Lopez-Nevot, Miguel A
Filiación: [Lopez-Escamez,JA; Gazquez,I] Otology & Neurotology Group CTS495, Department of Otolaryngology, Hospital de Poniente, El Ejido, Almería, Spain. [Lopez-Escamez,JA] GENYO, - Centro de Genómica e Investigación Oncológica - Pfizer/Universidad de Granada/Junta de Andalucía, Granada, Spain. [Saenz-Lopez,P; Gazquez,I; Moreno,A; Lopez-Nevot,MA] Department of Immunology, Hospital Universitario Virgen de las Nieves, Granada, Spain. [Gonzalez-Oller,C; Ibañez,A] Otology & Neurotology Group CTS495, Department of Biotechnology, Hospital de Poniente, El Ejido, Almeria. [Soto-Varela,A; Santos,S] Division of Otoneurology, Department of Otorhinolaryngology, Complejo Hospitalario Universitario, Santiago de Compostela, Spain. [Aran,I] Department of Otolaryngology, Complejo Hospitalario de Pontevedra, Spain. [Perez-Garrigues] Division Otoneurology, Department of Otorhinolaryngology, Hospital La Fe, Valencia, Spain
Grupo de Investigación: CTS 495 Otologia y Otoneurologia
MeSH: Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antigen-Antibody Complex
Medical Subject Headings::Diseases::Immune System Diseases::Autoimmune Diseases
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies
Medical Subject Headings::Diseases::Otorhinolaryngologic Diseases::Ear Diseases::Labyrinth Diseases::Endolymphatic Hydrops::Meniere Disease
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism, Genetic::Polymorphism, Single Nucleotide
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Fc::Receptors, IgG
Fecha de publicación : Jan-2011
Editorial : Biomed Central
Cita Bibliográfica: Lopez-Escamez JA, Saenz-Lopez P, Gazquez I, Moreno A, Gonzalez-Oller C, Soto-Varela A, et al.Polymorphisms of CD16A and CD32 Fcgamma receptors and circulating immune complexes in Meniere's disease: a case-control study. BMC Med Genet. 2011;12:2
Abstract: BACKGROUND: Autoimmune diseases with elevated circulating autoantibodies drive tissue damage and the onset of disease. The Fcγ receptors bind IgG subtypes modulating the clearance of circulating immune complexes (CIC). The inner ear damage in Ménière's disease (MD) could be mediated by an immune response driven by CIC. We examined single-nucleotide polymorphism (SNPs) in the CD16A and CD32 genes in patients with MD which may determine a Fcγ receptor with lower binding to CIC. METHODS: The functional CD16A (FcγRIIIa*559A > C, rs396991) and CD32A (FcγRIIa*519A > G, rs1801274) SNPs were analyzed using PCR-based TaqMan Genotyping Assay in two cohorts of 156 mediterranean and 112 Galicia patients in a case-control study. Data were analyzed by χ2 with Fisher's exact test and Cochran-Armitage trend test (CATT). CIC were measured by ELISA for C1q-binding CIC. RESULTS: Elevated CIC were found in 7% of patients with MD during the intercrisis period. No differences were found in the allelic frequency for rs396991 or rs1801274 in controls subjects when they were compared with patients with MD from the same geographic area. However, the frequency of AA and AC genotypes of CD16A (rs396991) differed among mediterranean and Galicia controls (Fisher's test, corrected p = 6.9 × 10-4 for AA; corrected p = 0.02 for AC). Although genotype AC of the CD16A receptor was significantly more frequent in mediterranean controls than in patients, [Fisher's test corrected p = 0.02; OR = 0.63 (0.44-0.91)], a genetic additive effect for the allele C was not observed (CATT, p = 0.23). Moreover, no differences were found in genotype frequencies for rs396991 between patients with MD and controls from Galicia (CATT, p = 0.14). The allelic frequency of CD32 (rs1801274) was not different between patients and controls either in mediterranean (p = 0.51) or Galicia population (p = 0.11). CONCLUSIONS: Elevated CIC are not found in most of patients with MD. Functional polymorphisms of CD16A and CD32 genes are not associated with onset of MD.
Descripción : This study was supported by a research project FIS PI07/0035. JALE was partially supported by ISCIII research grant INT09/229. The 3130 XL Genetics Analyzer was funded by grant IF06/37291 from Ministry of Science.
URI: http://hdl.handle.net/10668/422
Versión del editor : http://www.biomedcentral.com/1471-2350/12/2
DOI: 10.1186/1471-2350-12-2
ISSN : 1471-2350 (Online)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves
01- Artículos - GENYO. Centro Pfizer-Andalucía de Genómica e Investigación Oncológica
01- Artículos - APES Hospital de Poniente

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