Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/467
Título : Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories
Autor : Martín, Ana
Herranz, Marta
Martínez Lirola, Miguel
Fernández Fernández, Rosa
Bouza, Emilio
García de Viedma, Darío
Filiación: [Martín,A; Herránz,M; Bouza,E; García de Viedma,D] Servicio de Microbiología Clínica y Enfermedades Infecciosas. Hospital Gregorio Marañón. Universidad Complutense. Madrid.[Martínez Lirola,M] Servicio de Microbiología, Complejo Hospitalario Torrecárdenas, Almería. [Fernández Fernández,R] Unidad de Tuberculosis del Distrito Poniente, Almería, Spain
Grupo de Investigación: INDAL-TB group
Palabras clave : Tuberculosis
Errores Diagnósticos
Estudios Prospectivos
Reacciones Falso Positivas
MeSH: Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Errors::False Positive Reactions
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Interspersed Repetitive Sequences
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Tandem Repeat Sequences::Minisatellite Repeats
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism, Genetic::Polymorphism, Restriction Fragment Length
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Specimen Handling
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Errors
Fecha de publicación : 14-Feb-2008
Editorial : BMC Microbiology
Cita Bibliográfica: Martín A, Herranz M, Martínez Lirola M, Fernández Fernández R, Bouza E, García de Viedma D. Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories. BMC Microbiol.; 8:30
Abstract: BACKGROUND. The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identification of the same MTB strain cultured from both the potential source of the contamination and from the false-positive candidate. The molecular tool usually applied in this context is IS6110-RFLP which takes a long time to provide an answer, usually longer than is acceptable for microbiologists and clinicians to make decisions. Our purpose in this study is to evaluate a novel PCR-based method, MIRU-VNTR as an alternative to assure a rapid and optimized analysis of cross-contamination alerts. RESULTS. MIRU-VNTR was prospectively compared with IS6110-RFLP for clarifying 19 alerts of false positivity from other laboratories. MIRU-VNTR highly correlated with IS6110-RFLP, reduced the response time by 27 days and clarified six alerts unresolved by RFLP. Additionally, MIRU-VNTR revealed complex situations such as contamination events involving polyclonal isolates and a false-positive case due to the simultaneous cross-contamination from two independent sources. CONCLUSION. Unlike standard RFLP-based genotyping, MIRU-VNTR i) could help reduce the impact of a false positive diagnosis of TB, ii) increased the number of events that could be solved and iii) revealed the complexity of some cross-contamination events that could not be dissected by IS6110-RFLP.
Descripción : INDAL-TB group: MI Sánchez, MC Rogado, T Cabezas, W SánchezYebra, J Martínez, MA Lucerna, P Barroso, I Cabeza- Barrera, LF Díez, M Rodríguez, M Escámez, P Marín, A Lazo, J Gamir, J Vázquez, C Gutiérrez, A Reyes and T Peñafiel. Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/467
Versión del editor : http://www.biomedcentral.com/1471-2180/8/30/abstract
DOI: 10.1186/1471-2180-8-30
ISSN : 1471-2180
Appears in Collections:01- Artículos - DAP Poniente de Almería
01- Artículos - Complejo Hospitalario Torrecárdenas

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