Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/474
Título : An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex.
Autor : Arilla, M C
Ibarrola, I
Martínez, A
Monteseirín, J
Conde, J
Asturias, J A
Filiación: [Arilla,MC;Ibarrola,I;Martínez,A;Asturias,JA]Research & Development Department,Bial-Arístegui, Bilbao, Spain.[Monteseirín,J] Department of Medicine, Immunology and Allergy Service, Virgen Macarena University Hospital, Sevilla.[Monteseirín,J;Conde,J] Sagrado Corazón Clinic, Sevilla, Spain
Palabras clave : Anisakis simplex
Anisakis allergy
Quantification
Monoclonal antibody
Immunoassay
Food allergy
Recombinant antigen
Parasite detection
Alergia a Anisakis
Cuantificación
Anticuerpo monoclonal
Inmunoensayo
Alergia a los alimentos
Antígeno recombinante
Detección de parásitos
MeSH: Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergens
Medical Subject Headings::Diseases::Parasitic Diseases::Intestinal Diseases, Parasitic::Anisakiasis
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Helminth
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Calcium-Binding Proteins
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunologic Tests::Immunoassay::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assay
Medical Subject Headings::Diseases::Animal Diseases::Fish Diseases
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Helminth Proteins
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Clone Cells::Hybridomas
Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Laboratory::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred BALB C
Medical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Mollusca
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificity
Medical Subject Headings::Anatomy::Hemic and Immune Systems::Immune System::Lymphatic System::Lymphoid Tissue::Spleen
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergens
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Helminth
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Helminth Proteins
Medical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Helminths::Nematoda::Secernentea::Ascaridida::Ascaridoidea::Anisakis
Medical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Arthropods::Crustacea
Fecha de publicación : 1-May-2008
Editorial : Cambridge University Press
Cita Bibliográfica: Arilla MC, Ibarrola I, Martínez A, Monteseirín J, Conde J, Asturias JA. An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex. Parasitology. 2008;135(6):735-40
Abstract: Anisakis simplex is a nematode parasite that can infect humans who have eaten raw or undercooked seafood. Larvae invading the gastrointestinal mucosa excrete/secrete proteins that are implicated in the pathogenesis of anisakiasis and can induce IgE-mediated symptoms. Since Ani s 1 is a potent secreted allergen with important clinical relevance, its measurement could assess the quality of allergenic products used in diagnosis/immunotherapy of Anisakis allergy and track the presence of A. simplex parasites in fish foodstuffs. An antibody-based ELISA for quantification of Ani s 1 has been developed based on monoclonal antibody 4F2 as capture antibody and biotin-labelled polyclonal antibodies against Ani s 1 as detection reagent. The dose-response standard curves, obtained with natural and recombinant antigens, ranged from 4 to 2000 ng/ml and were identical and parallel to that of the A. simplex extract. The linear portion of the dose-response curve with nAni s 1 was between 15 and 250 ng/ml with inter-assay and intra-assays coefficients of variation less than 20% and 10%, respectively. The assay was specific since there was no cross-reaction with other extracts (except Ascaris extracts) and was highly sensitive (detection limit of 1·8 ng/ml), being able to detect Ani s 1 in fish extracts from codfish and monkfish.
URI: http://hdl.handle.net/10668/474
Versión del editor : http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1886152
DOI: 10.1017/S0031182008004332
ISSN : 1469-8161 (Print)
0031-1820 (Online)
Appears in Collections:01- Artículos - Hospital Virgen Macarena

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