Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/631
Título : Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases.
Autor : Alonso-Rodríguez, Noelia
Martínez-Lirola, Miguel
Sánchez, M Luisa
Herranz, Marta
Peñafiel, Teresa
Bonillo, Magdalena del Carmen
Gonzalez-Rivera, Milagros
Martínez, Juan
Cabezas, Teresa
Diez-García, Luis Felipe
Bouza, Emilio
García de Viedma, Darío
Filiación: [Alonso-Rodriguez,N; Herranz,M; Bouza,E; García de Viedma,D] Servicio de Microbiología, Hospital Gregorio Marañón, Madrid, Spain. [Herranz,M; Bouza,E] CIBER Enfermedades Respiratorias (CIBERES), Spain. [Martínez-Lirola,M; Diez-García,LF] Complejo Hospitalario Torrecárdenas, Almería, Spain. [Cabezas,T] Empresa Publica Hospital de Poniente, El Ejido, Almería, Spain. [Bonillo, MC]Distrito Atención Primaría Levante-Alto Almanzora, Huercal-Overa, Almería, Spain. [Sánchez,ML; Peñafiel,T] Unidad de Tuberculosis de Poniente, El Ejido, Almería, Spain. [Gonzalez-Rivera,M]LI Secuenciación, Hospital General Universitario Gregorio Marañón, Madrid, Spain. [Martínez,J] Centro Penitenciario El Acebuche, Almería, Spain
Palabras clave : Técnicas de Tipificación Bacteriana
Análisis por Conglomerados
Dermatoglifos de ADN
ADN bacteriano
Genotipo
Secuencias Repetitivas Esparcidas
Repeticiones de Minisatélite
epidemiología molecular
Mycobacterium tuberculosis
Reacción en Cadena de la Polimerasa
Polimorfismo de Longitud del Fragmento de Restricción
Tuberculosis
MeSH: Medical Subject Headings::Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Statistics as Topic::Cluster Analysis
Medical Subject Headings::Disciplines and Occupations::Social Sciences::Criminology::Forensic Sciences::Forensic Medicine::DNA Fingerprinting
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Interspersed Repetitive Sequences
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Tandem Repeat Sequences::Minisatellite Repeats
Medical Subject Headings::Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Molecular Epidemiology
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism, Genetic::Polymorphism, Restriction Fragment Length
Medical Subject Headings::Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificity
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques::Bacterial Typing Techniques
Fecha de publicación : 20-May-2009
Editorial : American Society for Microbiology
Cita Bibliográfica: Alonso-Rodriguez N, Martínez-Lirola M, Sánchez ML, Herranz M, Peñafiel T, Bonillo M del C, et al. Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases. J. Clin. Microbiol. 2009 Jul; 47(7):2026-32
Abstract: The use of molecular tools for genotyping Mycobacterium tuberculosis isolates in epidemiological surveys in order to identify clustered and orphan strains requires faster response times than those offered by the reference method, IS6110 restriction fragment length polymorphism (RFLP) genotyping. A method based on PCR, the mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping technique, is an option for fast fingerprinting of M. tuberculosis, although precise evaluations of correlation between MIRU-VNTR and RFLP findings in population-based studies in different contexts are required before the methods are switched. In this study, we evaluated MIRU-VNTR genotyping (with a set of 15 loci [MIRU-15]) in parallel to RFLP genotyping in a 39-month universal population-based study in a challenging setting with a high proportion of immigrants. For 81.9% (281/343) of the M. tuberculosis isolates, both RFLP and MIRU-VNTR types were obtained. The percentages of clustered cases were 39.9% (112/281) and 43.1% (121/281) for RFLP and MIRU-15 analyses, and the numbers of clusters identified were 42 and 45, respectively. For 85.4% of the cases, the RFLP and MIRU-15 results were concordant, identifying the same cases as clustered and orphan (kappa, 0.7). However, for the remaining 14.6% of the cases, discrepancies were observed: 16 of the cases clustered by RFLP analysis were identified as orphan by MIRU-15 analysis, and 25 cases identified as orphan by RFLP analysis were clustered by MIRU-15 analysis. When discrepant cases showing subtle genotypic differences were tolerated, the discrepancies fell from 14.6% to 8.6%. Epidemiological links were found for 83.8% of the cases clustered by both RFLP and MIRU-15 analyses, whereas for the cases clustered by RFLP or MIRU-VNTR analysis alone, links were identified for only 30.8% or 38.9% of the cases, respectively. The latter group of cases mainly comprised isolates that could also have been clustered, if subtle genotypic differences had been tolerated. MIRU-15 genotyping seems to be a good alternative to RFLP genotyping for real-time interventional schemes. The correlation between MIRU-15 and IS6110 RFLP findings was reasonable, although some uncertainties as to the assignation of clusters by MIRU-15 analysis were identified.
URI: http://hdl.handle.net/10668/631
Versión del editor : http://jcm.asm.org/content/47/7/2026
DOI: 10.1128/JCM.02308-08
ISSN : 1098-660X (Online)
0095-1137 (Print)
Appears in Collections:01- Artículos - AGS Norte de Almería
01- Artículos - APES Hospital de Poniente
01- Artículos - Complejo Hospitalario Torrecárdenas

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