Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/644
Título : The immunomodulator PSK induces in vitro cytotoxic activity in tumour cell linesvia arrest of cell cycle and induction of apoptosis
Autor : Jiménez-Medina, Eva
Berruguilla, Enrique
Romero, Irene
Algarra, Ignacio
Collado, Antonia
Garrido, Federico
Garcia-Lora, Angel
Filiación: [Jiménez-Medina,E; Berruguilla,E; Romero,I; Garrido,F; Garcia-Lora,A] Servicio de Análisis Clínicos e Inmunologia, Hospital Universitario Virgen de las Nieves, Universidad de Granada, Granada, Spain. [Algarra, I] Departamento de Ciencias de la Salud, Universidad Jaén, Jaén, Spain. [Collado, A] Unidad de Investigación, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Palabras clave : Animales
Apoptosis
Ciclo Celular
Línea Celular
Proliferación de la Célula
Ensayos de Selección de Medicamentos Antitumorales
Proteínas Fúngicas
Hongos
Células HeLa
Humanos
Factores Inmunológicos
Células Jurkat
Linfocitos
Melanoma Experimental
Ratones
Polisacáridos
Proteoglicanos
MeSH: Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Drug Screening Assays, Antitumor
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins
Medical Subject Headings::Organisms::Eukaryota::Fungi
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor::HeLa Cells
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Immunologic Factors
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor::Jurkat Cells
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal::Disease Models, Animal::Neoplasms, Experimental::Melanoma, Experimental
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::Proteoglycans
Fecha de publicación : 24-Mar-2008
Editorial : BioMed Central
Cita Bibliográfica: Jiménez-Medina E, Berruguilla E, Romero R ,Algarra I, Collado A, Garrido F.The immunomodulator PSK induces in vitro cytotoxic activity in tumour cell lines via arrest of cell cycle and induction of apoptosis.BMC Cancer. 2008 Mar 24;8:78
Abstract: BACKGROUND Protein-bound polysaccharide (PSK) is derived from the CM-101 strain of the fungus Coriolus versicolor and has shown anticancer activity in vitro and in in vivo experimental models and human cancers. Several randomized clinical trials have demonstrated that PSK has great potential in adjuvant cancer therapy, with positive results in the adjuvant treatment of gastric, esophageal, colorectal, breast and lung cancers. These studies have suggested the efficacy of PSK as an immunomodulator of biological responses. The precise molecular mechanisms responsible for its biological activity have yet to be fully elucidated. METHODS The in vitro cytotoxic anti-tumour activity of PSK has been evaluated in various tumour cell lines derived from leukaemias, melanomas, fibrosarcomas and cervix, lung, pancreas and gastric cancers. Tumour cell proliferation in vitro was measured by BrdU incorporation and viable cell count. Effect of PSK on human peripheral blood lymphocyte (PBL) proliferation in vitro was also analyzed. Studies of cell cycle and apoptosis were performed in PSK-treated cells. RESULTS PSK showed in vitro inhibition of tumour cell proliferation as measured by BrdU incorporation and viable cell count. The inhibition ranged from 22 to 84%. Inhibition mechanisms were identified as cell cycle arrest, with cell accumulation in G0/G1 phase and increase in apoptosis and caspase-3 expression. These results indicate that PSK has a direct cytotoxic activity in vitro, inhibiting tumour cell proliferation. In contrast, PSK shows a synergistic effect with IL-2 that increases PBL proliferation. CONCLUSION These results indicate that PSK has cytotoxic activity in vitro on tumour cell lines. This new cytotoxic activity of PSK on tumour cells is independent of its previously described immunomodulatory activity on NK cells.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/644
Versión del editor : http://www.biomedcentral.com/1471-2407/8/78
DOI: 10.1186/1471-2407-8-78
ISSN : 1471-2407 (Online)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves

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