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Título : Breast cancer 1 (BrCa1) may be behind decreased lipogenesis in adipose tissue from obese subjects.
Autor : Ortega, Francisco J
Moreno-Navarrete, José M
Mayas Torres, María Dolores
García-Santos, Eva
Gómez-Serrano, María
Rodríguez-Hermosa, José I
Ruiz, Bartomeu
Ricart, Wifredo
Tinahones Madueño, Francisco José
Frühbeck, Gema
Peral, Belén
Fernández-Real, José M
Filiación: [Ortega,FJ; Moreno-Navarrete,JM; Fernández-Real,JM] Service of Diabetes, Endocrinology and Nutrition, Institut d'Investigació Biomèdica de Girona, Girona, Spain. [Mayas Torres,MD; Tinahones Madueño,FJ] Service of Endocrinology and Nutrition, Hospital Universitario Virgen de Victoria, Málaga, Spain. [García-Santos,E; Gómez-Serrano,M; Peral,B] Department of Endocrinology, Physiopathology and Nervous System, Instituto de Investigaciones Biomédicas ‘Alberto Sols’, Consejo Superior de Investigaciones Científicas. Universidad Autónoma de Madrid, Madrid, Spain. [Rodríguez-Hermosa,JI; Ruiz,B] Department of Surgery, Institut d'Investigació Biomèdica de Girona, Girona, Spain. [Frühbeck,G] Department of Endocrinology, Clínica Universidad de Navarra, Navarra, Spain.
Palabras clave : RNA, Messenger
Acetyl-CoA Carboxylase
BRCA1 Protein
ARN Mensajero
Acetil CoA Carboxilasa
Proteína BRCA1
Tejido Adiposo
Diferenciación Celular
Fosforilación oxidativa
Células 3T3-L1
MeSH: Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Ligases::Carbon-Carbon Ligases::Acetyl-CoA Carboxylase
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Neoplasm Proteins::Tumor Suppressor Proteins::BRCA1 Protein
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Lipogenesis
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Macrophages
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Phosphorylation::Oxidative Phosphorylation
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messenger
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::3T3 Cells::Swiss 3T3 Cells::3T3-L1 Cells
Fecha de publicación : 30-May-2012
Editorial : Public Library of Science
Cita Bibliográfica: Ortega FJ, Moreno-Navarrete JM, Mayas Torres MD, García-Santos E, Gómez-Serrano M, Rodriguez-Hermosa JI, et al. Breast cancer 1 (BrCa1) may be behind decreased lipogenesis in adipose tissue from obese subjects. PLoS ONE; 7(5):e33233
Abstract: CONTEXT Expression and activity of the main lipogenic enzymes is paradoxically decreased in obesity, but the mechanisms behind these findings are poorly known. Breast Cancer 1 (BrCa1) interacts with acetyl-CoA carboxylase (ACC) reducing the rate of fatty acid biosynthesis. In this study, we aimed to evaluate BrCa1 in human adipose tissue according to obesity and insulin resistance, and in vitro cultured adipocytes. RESEARCH DESIGN AND METHODS BrCa1 gene expression, total and phosphorylated (P-) BrCa1, and ACC were analyzed in adipose tissue samples obtained from a total sample of 133 subjects. BrCa1 expression was also evaluated during in vitro differentiation of human adipocytes and 3T3-L1 cells. RESULTS BrCa1 gene expression was significantly up-regulated in both omental (OM; 1.36-fold, p = 0.002) and subcutaneous (SC; 1.49-fold, p = 0.001) adipose tissue from obese subjects. In parallel with increased BrCa1 mRNA, P-ACC was also up-regulated in SC (p = 0.007) as well as in OM (p = 0.010) fat from obese subjects. Consistent with its role limiting fatty acid biosynthesis, both BrCa1 mRNA (3.5-fold, p<0.0001) and protein (1.2-fold, p = 0.001) were increased in pre-adipocytes, and decreased during in vitro adipogenesis, while P-ACC decreased during differentiation of human adipocytes (p = 0.005) allowing lipid biosynthesis. Interestingly, BrCa1 gene expression in mature adipocytes was restored by inflammatory stimuli (macrophage conditioned medium), whereas lipogenic genes significantly decreased. CONCLUSIONS The specular findings of BrCa1 and lipogenic enzymes in adipose tissue and adipocytes reported here suggest that BrCa1 might help to control fatty acid biosynthesis in adipocytes and adipose tissue from obese subjects.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
Versión del editor :
DOI: 10.1371/journal.pone.0033233
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Virgen de la Victoria

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