Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/683
Título : Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering
Autor : Sanchez-Quevedo, M.C.
Alaminos, M.
Capitan, L.M.
Moreu, G.
Garzon, I.
Crespo, P.V.
Campos, A
Filiación: [Sanchez-Quevedo,MC; Alaminos,M; Garzon,I; Crespo,PV; Campos,A.] Department of Histology, University of Granada, Spain. [Alaminos,M] Fundación Hospital Clínico, Granada, Spain.[Capitan,LM] Division of Oral and Maxillofacial Surgery, University Hospital Virgen de las Nieves, Granada, Spain. [Moreu,G] Department of Stomatology, University of Granada, Spain.
Palabras clave : Técnicas de Cultivo de Célula
Supervivencia Celular
Células Cultivadas
Microanálisis por Sonda Electrónica
Fibrina
Fibroblastos
Expresión Génica
Perfilación de la Expresión Génica
Humanos
Hidrogeles
Inmunohistoquímica
Queratinocitos
Microscopía Electrónica
Mucosa Bucal
Análisis de Secuencia en Orden de Oligonucleótido
Sefarosa
Ingeniería de Tejidos
Fibrin-agarose
Constructs
Tissue engineering
MeSH: Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival
Medical Subject Headings::Anatomy::Cells::Cells, Cultured
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Electron::Electron Probe Microanalysis
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Fibrin
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Fibroblasts
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Expression Profiling
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Complex Mixtures::Colloids::Gels::Hydrogels
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Histocytochemistry::Immunohistochemistry
Medical Subject Headings::Anatomy::Cells::Epithelial Cells::Keratinocytes
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy, Electron
Medical Subject Headings::Anatomy::Tissues::Membranes::Mucous Membrane::Mouth Mucosa
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Microchip Analytical Procedures::Microarray Analysis::Oligonucleotide Array Sequence Analysis
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharose
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering
Fecha de publicación : Jun-2007
Editorial : Universidad de Murcia
Cita Bibliográfica: Sanchez-Quevedo MC, Alaminos M, Capitan LM, Moreu G, Garzon I et al.Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007 Jun;22(6):631-40
Abstract: Reconstruction of large oral mucosa defects is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering using a fibrin-agarose scaffold. For that purpose, we generated primary cultures of human oral mucosa fibroblasts and keratinocytes from small biopsies of normal oral mucosa using enzymatic treatments. Then we determined the viability of the cultured cells by electron probe quantitative X-ray microanalysis, and we demonstrated that most of the cells in the primary cultures were alive and had high K/Na ratios. Once cell viability was determined, we used the cultured fibroblasts and keratinocytes to develop an artificial oral mucosa construct by using a fibrin-agarose extracellular matrix and a sequential culture technique using porous culture inserts. Histological analysis of the artificial tissues showed high similarities with normal oral mucosa controls. The epithelium of the oral substitutes had several layers, with desmosomes and apical microvilli and microplicae. Both the controls and the oral mucosa substitutes showed high suprabasal expression of cytokeratin 13 and low expression of cytokeratin 10. All these results suggest that our model of oral mucosa using fibrin-agarose scaffolds show several similarities with native human oral mucosa.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/683
Versión del editor : http://www.hh.um.es/Abstracts/Vol_22/22_6/22_6_631.htm
ISSN : 0213-3911 (Print)
1699-5848 (Online)
Appears in Collections:01- Artículos - Hospital San Cecilio
01- Artículos - Hospital Virgen de las Nieves

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