Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/689
Title: Intratracheal dopamine attenuates pulmonary edema and improves survival after ventilator-induced lung injury in rats
Authors: Chamorro-Marín, Virginia
García-Delgado, Manuel
Touma-Fernández, Angel
Aguilar-Alonso, Eduardo
Fernández-Mondejar, Enrique
metadata.dc.contributor.authoraffiliation: [Chamorro-Marín,V] Unidad Experimental, Hospital Universitario Virgen de las Nieves, Granada, Spain.[García-Delgado,M; Touma-Fernández,A; Aguilar-Alonso,E; Fernández-Mondejar,E] Servicio de Cuidados Críticos y Urgencias, Hospital Universitario Virgen de las Nieves, Granada, Spain.[Chamorro-Marín,V; García-Delgado,M; Touma-Fernández,A; Aguilar-Alonso,E; Fernández-Mondejar,E] Servicio de Cuidados Críticos y Urgencias, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Keywords: Análisis de Varianza;Animales;Dopamina beta-Hidroxilasa;Lesión Pulmonar;Masculino;Edema Pulmonar;Distribución Aleatoria;Ratas;Ratas Wistar;Respiración Artificial;Tasa de Supervivencia;Tráquea
metadata.dc.subject.mesh: Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Biogenic Amines::Biogenic Monoamines::Catecholamines::Dopamine
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Lung Diseases::Lung Injury
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Lung Diseases::Pulmonary Edema
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Random Allocation
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats, Wistar
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Airway Management::Respiration, Artificial
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Vital Statistics::Mortality::Survival Rate
Medical Subject Headings::Anatomy::Respiratory System::Trachea
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Analysis of Variance
Issue Date: 10-Mar-2008
Publisher: BioMed Central
Citation: Chamorro-Marín V, García-Delgado M, Touma-Fernández A, Aguilar-Alonso E, Fernández-Mondejar,EIntratracheal dopamine attenuates pulmonary edema andimproves survival after ventilator-induced lung injury in rats. Crit Care. 2008;12(2):R39.
Abstract: INTRODUCTION Clearance of alveolar oedema depends on active transport of sodium across the alveolar-epithelial barrier. beta-Adrenergic agonists increase clearance of pulmonary oedema, but it has not been established whether beta-agonist stimulation achieves sufficient oedema clearance to improve survival in animals. The objective of this study was to determine whether the increased pulmonary oedema clearance produced by intratracheal dopamine improves the survival of rats after mechanical ventilation with high tidal volume (HVT). METHODS This was a randomized, controlled, experimental study. One hundred and thirty-two Wistar-Kyoto rats, weighing 250 to 300 g, were anaesthetized and cannulated via endotracheal tube. Pulmonary oedema was induced by endotracheal instillation of saline solution and mechanical ventilation with HVT. Two types of experiment were carried out. The first was an analysis of pulmonary oedema conducted in six groups of 10 rats ventilated with low (8 ml/kg) or high (25 ml/kg) tidal volume for 30 or 60 minutes with or without intratracheally instilled dopamine. At the end of the experiment the animals were exsanguinated and pulmonary oedema analysis performed. The second experiment was a survival analysis, which was conducted in two groups of 36 animals ventilated with HVT for 60 minutes with or without intratracheal dopamine; survival of the animals was monitored for up to 7 days after extubation. RESULTS In animals ventilated at HVT with or without intratracheal dopamine, oxygen saturation deteriorated over time and was significantly higher at 30 minutes than at 60 minutes. After 60 minutes, a lower wet weight/dry weight ratio was observed in rats ventilated with HVT and instilled with dopamine than in rats ventilated with HVT without dopamine (3.9 +/- 0.27 versus 4.9 +/- 0.29; P = 0.014). Survival was significantly (P = 0.013) higher in animals receiving intratracheal dopamine and ventilated with HVT, especially at 15 minutes after extubation, when 11 of the 36 animals in the HVT group had died as compared with only one out of the 36 animals in the HVT plus dopamine group. CONCLUSION Intratracheal dopamine instillation increased pulmonary oedema clearance in rats ventilated with HVT, and this greater clearance was associated with improved survival.
Description: Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/689
metadata.dc.relation.publisherversion: http://ccforum.com/content/12/2/R39
metadata.dc.identifier.doi: 10.1186/cc6829
ISSN: 1364-8535 (Print)
1466-609X (Online)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves

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