Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/694
Title: Adipose tissue gene expression of factors related to lipid processing in obesity.
Authors: Clemente-Postigo, Mercedes
Queipo-Ortuño, María Isabel
Fernández-García, Diego José
Gómez-Huelgas, Ricardo
Tinahones Madueño, Francisco José
Cardona Díaz, Fernando
metadata.dc.contributor.authoraffiliation: [Clemente-Postigo,M; Queipo-Ortuño,MI; Fernández-García,DJ] Laboratorio de Investigaciones Biomédicas del Hospital Universitario Virgen de la Victoria, Málaga (Fundación IMABIS), Spain. [Queipo-Ortuño,MI; Fernández-García,DJ; Gomez-Huelgas,R; Tinahones FJ; Cardona,F] CIBER Fisiopatología de la Obesidad y la Nutrición, Spain. [Fernández-García,DJ; Tinahones Madueño,FJ] Servicio Endocrinología y Nutrición del Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Gómez-Huelgas,R] Servicio de Medicina Interna del Hospital Regional Carlos Haya, Spain.
Keywords: LRP1 protein, human;acylation stimulating protein, human;Proteínas de Unión a Ácidos Grasos;Índice de masa corporal;Diabetes Mellitus Tipo 2;FABP4 protein, human;Expresión genética;Resistencia a la insulina;Péptidos y Proteínas de Señalización Intercelular;Grasa intraabdominal;Metabolismo de los Lípidos;Lipoproteína Lipasa;Proteina 1 Relacionada con Receptor de Lipoproteína de Baja Densidad;Obesidad mórbida;Reacción en Cadena de la Polimerasa de Transcriptasa Inversa;Grasa subcutánea
metadata.dc.subject.mesh: Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Mass Index
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus::Diabetes Mellitus, Type 2
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding Proteins
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance::Insulin Resistance
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Abdominal Fat::Intra-Abdominal Fat
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Carboxylic Ester Hydrolases::Lipoprotein Lipase
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::LDL-Receptor Related Proteins::Low Density Lipoprotein Receptor-Related Protein-1
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity::Obesity, Morbid
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Subcutaneous Fat
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young Adult
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Issue Date: 22-Sep-2011
Publisher: Public Library of Science
Citation: Clemente-Postigo M, Queipo-Ortuño MI, Fernandez-Garcia DJ, Gomez-Huelgas R, Tinahones Madueño FJ, Cardona Díaz F. Adipose tissue gene expression of factors related to lipid processing in obesity. PLoS ONE; 6(9):e24783
Abstract: BACKGROUND Adipose tissue lipid storage and processing capacity can be a key factor for obesity-related metabolic disorders such as insulin resistance and diabetes. Lipid uptake is the first step to adipose tissue lipid storage. The aim of this study was to analyze the gene expression of factors involved in lipid uptake and processing in subcutaneous (SAT) and visceral (VAT) adipose tissue according to body mass index (BMI) and the degree of insulin resistance (IR). METHODS AND PRINCIPAL FINDINGS VLDL receptor (VLDLR), lipoprotein lipase (LPL), acylation stimulating protein (ASP), LDL receptor-related protein 1 (LRP1) and fatty acid binding protein 4 (FABP4) gene expression was measured in VAT and SAT from 28 morbidly obese patients with Type 2 Diabetes Mellitus (T2DM) or high IR, 10 morbidly obese patients with low IR, 10 obese patients with low IR and 12 lean healthy controls. LPL, FABP4, LRP1 and ASP expression in VAT was higher in lean controls. In SAT, LPL and FABP4 expression were also higher in lean controls. BMI, plasma insulin levels and HOMA-IR correlated negatively with LPL expression in both VAT and SAT as well as with FABP4 expression in VAT. FABP4 gene expression in SAT correlated inversely with BMI and HOMA-IR. However, multiple regression analysis showed that BMI was the main variable contributing to LPL and FABP4 gene expression in both VAT and SAT. CONCLUSIONS Morbidly obese patients have a lower gene expression of factors related with lipid uptake and processing in comparison with healthy lean persons.
Description: Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/694
metadata.dc.relation.publisherversion: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0024783
metadata.dc.identifier.doi: 10.1371/journal.pone.0024783
ISSN: 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Regional de Málaga
01- Artículos - Hospital Virgen de la Victoria
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

Files in This Item:
File Description SizeFormat 
Clemente_Adipose.pdfArtículo publicado173,16 kBAdobe PDFView/Open


This item is protected by original copyright



This item is licensed under a Creative Commons License Creative Commons