Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/698
Title: Aggravation of chronic stress effects on hippocampal neurogenesis and spatial memory in LPA₁ receptor knockout mice.
Authors: Castilla-Ortega, Estela
Hoyo-Becerra, Carolina
Pedraza, Carmen
Chun, Jerold
Rodríguez De Fonseca, Fernando
Estivill-Torrús, Guillermo
Santín, Luis J
metadata.dc.contributor.authoraffiliation: [Castilla-Ortega, E; Pedraza, C; Santín, LJ] Departamento de Psicobiología y Metodología de las CC, Universidad de Málaga, Málaga, Spain. [Hoyo-Becerra, C; Rodríguez de Fonseca, F; Estibill-Torrús, G] Unidad de Investigación, Fundación IMABIS, Hospital Regional Universitario Carlos Haya, Málaga, Spain. [Chun, J] Department of Molecular Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America.
Keywords: Animales;Ansiedad;recuento de células;proliferación celular;Supervivencia Celular;Conducta Exploratoria;técnicas de inactivación genética;Hipocampo;Masculino;Memoria;Ratones;proteínas asociadas a microtúbulos;células madre nerviosas;Neurogénesis;Neuronas;Neuroglía;tamaño de los órganos;receptores de ácidos lisofosfatídicos;restricción física;Conducta Espacial;Estrés Psicológico
metadata.dc.subject.mesh: Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Emotions::Anxiety
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Count
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival
Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Exploratory Behavior
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Targeting::Gene Knockout Techniques
Medical Subject Headings::Anatomy::Nervous System::Central Nervous System::Brain::Limbic System::Hippocampus
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Psychiatry and Psychology::Psychological Phenomena and Processes::Mental Processes::Learning::Memory
Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Neurobehavioral Manifestations::Memory Disorders
Medical Subject Headings::Psychiatry and Psychology::Psychological Phenomena and Processes::Mental Processes::Learning::Memory::Memory, Short-Term
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Cytoskeletal Proteins::Microtubule Proteins::Microtubule-Associated Proteins
Medical Subject Headings::Anatomy::Cells::Stem Cells::Neural Stem Cells
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation::Neurogenesis
Medical Subject Headings::Anatomy::Cells::Neuroglia
Medical Subject Headings::Anatomy::Nervous System::Neurons
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Neuropeptides
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Growth::Organ Size
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, G-Protein-Coupled::Receptors, Lysophospholipid::Receptors, Lysophosphatidic Acid
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immobilization::Restraint, Physical
Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Spatial Behavior
Medical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Behavioral Symptoms::Stress, Psychological
Medical Subject Headings::Organisms::Eukaryota::Animals
Issue Date: 29-Sep-2011
Publisher: Public Library of Science
Citation: Castilla-Ortega E, Hoyo-Becerra C, Pedraza C, Chun J, Rodríguez De Fonseca F, Estivill-Torrús G, et al. Aggravation of chronic stress effects on hippocampal neurogenesis and spatial memory in LPA₁ receptor knockout mice. PLoS ONE 2011; 6(9):e25522
Abstract: BACKGROUND The lysophosphatidic acid LPA₁ receptor regulates plasticity and neurogenesis in the adult hippocampus. Here, we studied whether absence of the LPA₁ receptor modulated the detrimental effects of chronic stress on hippocampal neurogenesis and spatial memory. METHODOLOGY/PRINCIPAL FINDINGS Male LPA₁-null (NULL) and wild-type (WT) mice were assigned to control or chronic stress conditions (21 days of restraint, 3 h/day). Immunohistochemistry for bromodeoxyuridine and endogenous markers was performed to examine hippocampal cell proliferation, survival, number and maturation of young neurons, hippocampal structure and apoptosis in the hippocampus. Corticosterone levels were measured in another a separate cohort of mice. Finally, the hole-board test assessed spatial reference and working memory. Under control conditions, NULL mice showed reduced cell proliferation, a defective population of young neurons, reduced hippocampal volume and moderate spatial memory deficits. However, the primary result is that chronic stress impaired hippocampal neurogenesis in NULLs more severely than in WT mice in terms of cell proliferation; apoptosis; the number and maturation of young neurons; and both the volume and neuronal density in the granular zone. Only stressed NULLs presented hypocortisolemia. Moreover, a dramatic deficit in spatial reference memory consolidation was observed in chronically stressed NULL mice, which was in contrast to the minor effect observed in stressed WT mice. CONCLUSIONS/SIGNIFICANCE These results reveal that the absence of the LPA₁ receptor aggravates the chronic stress-induced impairment to hippocampal neurogenesis and its dependent functions. Thus, modulation of the LPA₁ receptor pathway may be of interest with respect to the treatment of stress-induced hippocampal pathology.
Description: Formal Correction: Error in Funding Posted by PLoS_ONE_Group on 29 Nov 2011 at 22:27 GMT The complete funding information is: "This work was supported by grants from the Spanish Ministry of Education and Science (MEC SEJ2007-61187, MICINN PSI2010-16160 to LJS), Programme for Stabilization of Researchers and Research Technician and Intensification of Research Activity in the National Health System (I3SNS Programme to GET), the Human Frontier Science Programme (to JC, FRDF), Fund for Health Research, Carlos III Health Institute (FIS 02/1643, FIS PI07/0629, FIS PI10/02514 to GET), Red de Trastornos Adictivos RTA (RD06/001 to FRDF), Andalusian Ministry of Health and of Innovation, Science and Enterprise (CTS065 to GET; CTS433 to FRDF) and the US National Institutes of Health MH051699 and MH01723 (to JC). The author E. Castilla-Ortega has a FPU Grant from the Spanish Ministry of Education (AP-2006-02582). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript." Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/698
metadata.dc.relation.publisherversion: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0025522
metadata.dc.identifier.doi: 10.1371/journal.pone.0025522
ISSN: 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Regional de Málaga
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

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