Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/750
Título : Tumor necrosis-like weak inducer of apoptosis as a proinflammatory cytokine in human adipocyte cells: up-regulation in severe obesity is mediated by inflammation but not hypoxia.
Autor : Vendrell, Joan
Maymó-Masip, Elsa
Tinahones Madueño, Francisco José
García-España, Antonio
Megía, Ana
Caubet, Enric
García-Fuentes, Eduardo
Chacón, Matilde R
Filiación: [Vendrell,J; Maymó-Masip,E; Megía,A; Chacón,MR] Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas, Hospital Universitari de Tarragona Joan XXIII, Spain. [Vendrell,J; Maymó-Masip,E; Megía,A; Chacón,MR; García-España,A] IISPV (Institut d’Investigació Sanitaria “Pere Virgili”), Universitat Rovira i Virgili, Tarragona, Spain. [Tinahones Madueño,FJ; García-Fuentes,E] Centro de Investigación Biomédica en Red de Fisiopatología, Obesidad y Nutrición, Department of Obesity and Nutrition, Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Caubet,E] Department of Surgery, Hospital St. Pau i Sta Tecla, Tarragona, Spain.
Palabras clave : TNFSF12 protein, human
TWEAK receptor
Tejido Adiposo
Biopsia
Western Blotting
Línea Celular
Estudios de Cohortes
Citocinas
Diabetes Mellitus Tipo 2
Retículo Endoplásmico
Expresión Génica
Inflamación
Macrófagos
Obesidad Mórbida
Receptores del Factor de Necrosis Tumoral
Transducción de Señal
Factores de Necrosis Tumoral
Regulación hacia Arriba
Adipocitos
Anoxia
MeSH: Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Respiratory::Anoxia
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Surgical::Biopsy
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Blotting, Western
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line
Medical Subject Headings::Health Care::Health Care Quality, Access, and Evaluation::Quality of Health Care::Health Care Evaluation Mechanisms::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus::Diabetes Mellitus, Type 2
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Endoplasmic Reticulum
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Macrophages
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity::Obesity, Morbid
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Tumor Necrosis Factor
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Signal Transduction
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Tumor Necrosis Factors
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Up-Regulation
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes
Fecha de publicación : Jun-2010
Editorial : The Endocrine Society
Cita Bibliográfica: Vendrell J, Maymó-Masip E, Tinahones Madueño FJ, García-España A, Megía A, Caubet E, et al. Tumor necrosis-like weak inducer of apoptosis as a proinflammatory cytokine in human adipocyte cells: up-regulation in severe obesity is mediated by inflammation but not hypoxia. J. Clin. Endocrinol. Metab.. 2010; 95(6):2983-92
Abstract: CONTEXT Adipose tissue hypoxia and endoplasmic reticulum (ER) stress may link the presence of chronic inflammation and macrophage infiltration in severely obese subjects. We previously reported the up-regulation of TNF-like weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible 14 (Fn14) axis in adipose tissue of severely obese type 2 diabetic subjects. OBJECTIVES The objective of the study was to examine TWEAK and Fn14 adipose tissue expression in obesity, severe obesity, and type 2 diabetes in relation to hypoxia and ER stress. DESIGN In the obesity study, 19 lean, 28 overweight, and 15 obese nondiabetic subjects were studied. In the severe obesity study, 23 severely obese and 35 control subjects were studied. In the type 2 diabetes study, 11 type 2 diabetic and 36 control subjects were studied. The expression levels of the following genes were analyzed in paired samples of sc and visceral adipose tissue: Fn14, TWEAK, VISFATIN, HYOU1, FIAF, HIF-1a, VEGF, GLUT-1, GRP78, and XBP-1. The effect of hypoxia, inflammation, and ER stress on the expression of TWEAK and Fn14 was examined in human adipocyte and macrophage cell lines. RESULTS Up-regulation of TWEAK/Fn14 and hypoxia and ER stress surrogate gene expression was observed in sc and visceral adipose tissue only in our severely obese cohort. Hypoxia modulates TWEAK or Fn14 expression in neither adipocytes nor macrophages. On the contrary, inflammation up-regulated TWEAK in macrophages and Fn14 expression in adipocytes. Moreover, TWEAK had a proinflammatory effect in adipocytes mediated by the nuclear factor-kappaB and ERK but not JNK signaling pathways. CONCLUSIONS Our data suggest that TWEAK acts as a pro-inflammatory cytokine in the adipose tissue and that inflammation, but not hypoxia, may be behind its up-regulation in severe obesity.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/750
Versión del editor : http://jcem.endojournals.org/content/95/6/2983.long
DOI: 10.1210/jc.2009-2481
ISSN : 1945-7197 (Online)
0021-972X (Print)
Appears in Collections:01- Artículos - Hospital Virgen de la Victoria

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