Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/780
Título : A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering.
Autor : Garzón, Ingrid
Carriel, Victor
Marín-Fernández, Ana Belén
Oliveira, Ana Celeste
Garrido-Gómez, Juan
Campos, Antonio
Sánchez-Quevedo, María Del Carmen
Alaminos, Miguel
Filiación: [Garzón,I; Carriel,V; Oliveira,AC; Garrido-Gómez,J; Campos,A; Sánchez-Quevedo, MC; Alaminos,M] Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain.[Marín-Fernández,AB] Division of Oral and Maxillofacial surgery, University Hospital Virgen de las Nieves, Granada, Spain. [Garrido-Gómez,J] Division of Trauma and Orthopedic Surgery, University Hospital San Cecilio, Granada, Spain.
Palabras clave : Disco de la Articulación Temporomandibular
Condrocitos
Trasplante Autólogo
Células Cultivadas
Ingeniería de Tejidos
Expresión Génica
Supervivencia Celular
Proteínas Reguladoras de la Apoptosis
MeSH: Medical Subject Headings::Anatomy::Stomatognathic System::Temporomandibular Joint::Temporomandibular Joint Disc
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Chondrocytes
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Transplantation, Autologous
Medical Subject Headings::Anatomy::Cells::Cells, Cultured
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::Apoptosis Regulatory Proteins
Fecha de publicación : 18-Dec-2012
Editorial : Public Library of Science
Nuno M. Neves, University of Minho, Portugal
Cita Bibliográfica: Garzón I, Carriel V, Marín-Fernández AB, Oliveira AC, Garrido-Gómez J, Campos A, et al. A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering. PLoS ONE. 2012; 7(12):e51961
Abstract: Temporo-mandibular joint disc disorders are highly prevalent in adult populations. Autologous chondrocyte implantation is a well-established method for the treatment of several chondral defects. However, very few studies have been carried out using human fibrous chondrocytes from the temporo-mandibular joint (TMJ). One of the main drawbacks associated to chondrocyte cell culture is the possibility that chondrocyte cells kept in culture tend to de-differentiate and to lose cell viability under in in-vitro conditions. In this work, we have isolated human temporo-mandibular joint fibrochondrocytes (TMJF) from human disc and we have used a highly-sensitive technique to determine cell viability, cell proliferation and gene expression of nine consecutive cell passages to determine the most appropriate cell passage for use in tissue engineering and future clinical use. Our results revealed that the most potentially viable and functional cell passages were P5-P6, in which an adequate equilibrium between cell viability and the capability to synthesize all major extracellular matrix components exists. The combined action of pro-apoptotic (TRAF5, PHLDA1) and anti-apoptotic genes (SON, HTT, FAIM2) may explain the differential cell viability levels that we found in this study. These results suggest that TMJF should be used at P5-P6 for cell therapy protocols.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/780
Versión del editor : http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0051961
DOI: 10.1371/journal.pone.0051961
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital San Cecilio
01- Artículos - Hospital Virgen de las Nieves

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