Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/831
Título : Up-regulation of placental leptin by human chorionic gonadotropin.
Autor : Maymó, Julieta L.
Pérez-Pérez, Antonio
Sánchez-Margalet, Víctor
Dueñas, José L.
Calvo, Juan Carlos
Varone, Cecilia L.
Filiación: [Maymó,JL; Calvo,JC; Varone, CL] Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina. [Pérez Pérez,A; Sánchez-Margalet,V] Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla. [Dueñas,JL] Servicio de Ginecología y Obstetricia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
Palabras clave : Línea Celular Tumoral
Cesárea
Coriocarcinoma
Gonadotropina Coriónica
Parto Obstétrico
Femenino
Flavonoides
Humanos
Leptina
Placenta
Embarazo
Regiones Promotoras Genéticas
Proteínas Recombinantes
Trofoblastos
Regulación hacia Arriba
Androstadienos
MeSH: Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Obstetric Surgical Procedures::Delivery, Obstetric::Cesarean Section
Medical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Germ Cell and Embryonal::Trophoblastic Neoplasms::Choriocarcinoma
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Gonadotropins::Chorionic Gonadotropin
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Obstetric Surgical Procedures::Delivery, Obstetric
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Benzopyrans::Chromones::Flavonoids
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Adipokines::Leptin
Medical Subject Headings::Anatomy::Embryonic Structures::Placenta
Medical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Reproduction::Pregnancy
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Regulatory Sequences, Nucleic Acid::Promoter Regions, Genetic
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Recombinant Proteins
Medical Subject Headings::Anatomy::Cells::Endocrine Cells::Trophoblasts
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Up-Regulation
Medical Subject Headings::Chemicals and Drugs::Polycyclic Compounds::Steroids::Androstanes::Androstenes::Androstadienes
Fecha de publicación : Jan-2009
Editorial : Endocrine Society
Cita Bibliográfica: Maymó JL, Pérez Pérez A, Sánchez-Margalet V, Dueñas JL, Calvo JC, Varone CL. Up-regulation of placental leptin by human chorionic gonadotropin. Endocrinology. 2009; 150(1): 304-13
Abstract: Leptin, the 16,000 molecular weight protein product of the obese gene, was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, leptin has been suggested to be involved in other functions during pregnancy, particularly in placenta, in which it was found to be expressed. In the present work, we have found that recombinant human chorionic gonadotropin (hCG) added to BeWo choriocarcinoma cell line showed a stimulatory effect on endogenous leptin expression, when analyzed by Western blot. This effect was time and dose dependent. Maximal effect was achieved at hCG 100 IU/ml. Moreover, hCG treatment enhanced leptin promoter activity up to 12.9 times, evaluated by transient transfection with a plasmid construction containing different promoter regions and the reporter gene luciferase. This effect was dose dependent and evidenced with all the promoter regions analyzed, regardless of length. Similar results were obtained with placental explants, thus indicating physiological relevance. Because hCG signal transduction usually involves cAMP signaling, this pathway was analyzed. Contrarily, we found that dibutyryl cAMP counteracted hCG effect on leptin expression. Furthermore, cotransfection with the catalytic subunit of PKA and/or the transcription factor cAMP response element binding protein repressed leptin expression. Thereafter we determined that hCG effect could be partially blocked by pharmacologic inhibition of MAPK pathway with 50 microM PD98059 but not by the inhibition of the phosphatidylinositol 3-kinase pathway with 0.1 microm wortmannin. Moreover, hCG treatment promoted MAPK kinase and ERK1/ERK2 phosphorylation in placental cells. Finally, cotransfection with a dominant-negative mutant of MAPK blocked the hCG-mediated activation of leptin expression. In conclusion, we provide some evidence suggesting that hCG induces leptin expression in trophoblastic cells probably involving the MAPK signal transduction pathway.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/831
Versión del editor : http://endo.endojournals.org/content/150/1/304.abstract
DOI: 10.1210/en.2008-0522
ISSN : 0013-7227 (Print)
1945-7170 (Online)
Appears in Collections:01- Artículos - Hospital Virgen Macarena

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