Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/842
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dc.contributor.authorSuárez, Juan-
dc.contributor.authorRomero-Zerbo, Yanina-
dc.contributor.authorMárquez, Lucia-
dc.contributor.authorRivera, Patricia-
dc.contributor.authorIglesias, Mar-
dc.contributor.authorBermúdez-Silva, Francisco J.-
dc.contributor.authorAndreu, Montserrat-
dc.contributor.authorRodríguez de Fonseca, Fernando-
dc.date.accessioned2013-03-18T09:25:10Z-
dc.date.available2013-03-18T09:25:10Z-
dc.date.issued2012-05-25-
dc.identifier.citationSuárez J, Romero-Zerbo Y, Márquez L, Rivera P, Iglesias M, Bermúdez-Silva FJ, et al. Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids. PLoS ONE. 2012; 7(5):e37729es
dc.identifier.issn1932-6203 (Online)-
dc.identifier.otherPMC3360619-
dc.identifier.urihttp://hdl.handle.net/10668/842-
dc.descriptionJournal Article; Research Support, Non-U.S. Gov't;es
dc.description.abstractStudies in animal models and humans suggest anti-inflammatory roles on the N acylethanolamide (NAE)-peroxisome proliferators activated receptor alpha (PPARα) system in inflammatory bowel diseases. However, the presence and function of NAE-PPARα signaling system in the ulcerative colitis (UC) of humans remain unknown as well as its response to active anti-inflammatory therapies such as 5-aminosalicylic acid (5-ASA) and glucocorticoids. Expression of PPARα receptor and PPARα ligands-biosynthetic (NAPE-PLD) and -degrading (FAAH and NAAA) enzymes were analyzed in untreated active and 5-ASA/glucocorticoids/immunomodulators-treated quiescent UC patients compared to healthy human colonic tissue by RT-PCR and immunohistochemical analyses. PPARα, NAAA, NAPE-PLD and FAAH showed differential distributions in the colonic epithelium, lamina propria, smooth muscle and enteric plexus. Gene expression analysis indicated a decrease of PPARα, PPARγ and NAAA, and an increase of FAAH and iNOS in the active colitis mucosa. Immunohistochemical expression in active colitis epithelium confirmed a PPARα decrease, but showed a sharp NAAA increase and a NAPE-PLD decrease, which were partially restored to control levels after treatment. We also characterized the immune cells of the UC mucosa infiltrate. We detected a decreased number of NAAA-positive and an increased number of FAAH-positive immune cells in active UC, which were partially restored to control levels after treatment. NAE-PPARα signaling system is impaired during active UC and 5-ASA/glucocorticoids treatment restored its normal expression. Since 5-ASA actions may work through PPARα and glucocorticoids through NAE-producing/degrading enzymes, the use of PPARα agonists or FAAH/NAAA blockers that increases endogenous PPARα ligands may yield similar therapeutics advantages.es
dc.description.sponsorshipThis study was supported by grants to FRdF from the European Union’s 7th Framework Programme (Health-F2-2008-223713, REPROBESITY); the following grants from the Spanish Ministry of Science and Innovation (SAF2010-20521); National Institute of Health ‘‘Carlos III’’ (PI07/1226, PI07/0880 and PI 07/0953), Red de Trastornos Adictivos-UE-ERDF (RD06/0001/0000) and El Centro de Investigación Biomédica en Red de Fisiopatología de Obesidad y Nutrición; grants from the Consejería de Economía, Innovación y Ciencia de la Junta de Andalucía, UE/ERDF (CTS-433 and PI45403); and a grant from the Consejería de Salud de la Junta de Andalucía (PI0232/2008), Spain. FJBS holds a Miguel Servet research contracts CD07/00283, and JS holds a Sara Borrell postdoctoral contract CD08/00203, both from the National Institute of Health ‘‘Carlos III’’, Madrid, Spain.es
dc.language.isoenes
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPloS Onees
dc.subjectAmidohidrolasases
dc.subjectAntiinflamatorioses
dc.subjectColitis Ulcerosaes
dc.subjectExpresión Génicaes
dc.subjectGlucocorticoideses
dc.subjectPPAR alfaes
dc.subjectPPAR gammaes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adultes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Agedes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolaseses
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Inflammatory Agentses
dc.subject.meshMedical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Intestinal Diseases::Inflammatory Bowel Diseases::Colitis, Ulcerativees
dc.subject.meshMedical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Lower Gastrointestinal Tract::Intestine, Large::Colones
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Alcohols::Amino Alcohols::Ethanolamineses
dc.subject.meshMedical Subject Headings::Check Tags::Femalees
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expressiones
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Glucocorticoidses
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestinal Mucosaes
dc.subject.meshMedical Subject Headings::Check Tags::Malees
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Acids, Carbocyclic::Benzoates::Hydroxybenzoates::Salicylates::Aminosalicylic Acids::Aminosalicylic Acid::Mesalaminees
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxidoreductases Acting on CH-NH2 Group Donors::Amino Acid Oxidoreductases::Nitric Oxide Synthase::Nitric Oxide Synthase Type IIes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Peroxisome Proliferator-Activated Receptors::PPAR alphaes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Peroxisome Proliferator-Activated Receptors::PPAR gammaes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Agedes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young Adultes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adolescentes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Phosphoric Diester Hydrolases::Phospholipases::Phospholipase Des
dc.titleUlcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoidses
dc.typeinfo:eu-repo/semantics/articlees
dc.description.versionYeses
dc.identifier.pmid22662201-
dc.rights.accessRightsAcceso abiertoes
dc.identifier.doi10.1371/journal.pone.0037729-
dc.type.versioninfo:eu-repo/semantics/publishedes
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0037729es
dc.contributor.authoraffiliation[Suárez,J; Romero-Zerbo,Y; Rivera,P; Bermúdez-Silva,FJ; Rodríguez de Fonseca,F] Laboratorio de Medicina Regenerativa, Hospital Carlos Haya, Mediterranean Institute for the Advance of Biotechnology and Health Research Fundación, Málaga, Spain. [Márquez,L; Andreu,M] Department of Gastroenterology, Parc de Salut Mar, Universidad Autónoma, Barcelona, Spain. [Suárez,J; Bermúdez-Silva,FJ; Rodríguez de Fonseca,F] El Centro de Investigación Biomédica en Red de Fisiopatología de Obesidad y Nutrición, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación, Madrid, Spain. [Iglesias,M] Department of Pathology, Parc de Salut Mar, Universidad Autónoma, Barcelona, Spain.es
dc.type.subtypeArtículoes
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga

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