Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/845
Título : FABP4 Dynamics in Obesity: Discrepancies in Adipose Tissue and Liver Expression Regarding Circulating Plasma Levels.
Autor : Queipo-Ortuño, María Isabel
Escoté, Xavier
Ceperuelo-Mallafré, María Victoria
Garrido-Sánchez, Lourdes
Miranda, Merce
Clemente-Postigo, Mercedes
Pérez-Pérez, Rafael
Peral, Belén
Cardona Díaz, Fernando
Fernández-Real, José Manuel
Tinahones Madueño, Francisco José
Vendrell, Joan
Filiación: [Queipo-Ortuño,MI; Ceperuelo-Mallafré,MV; Clemente-Postigo,M; Cardona Díaz,F] Laboratorio de Investigaciones Biomédicas del Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Queipo-Ortuño,MI; Ceperuelo-Mallafré,MV; Clemente-Postigo,M; Cardona Díaz,F] Fundación IMABIS, Málaga, Spain. [Queipo-Ortuño,MI; Pérez-Pérez,R; Peral,B; Cardona Díaz,F; Fernández-Real,JM; Tinahones Madueño,FJ] CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. [Escoté,X; Garrido-Sánchez,L; Miranda,M; Vendrell,J] Endocrinology and Diabetes Unit.Joan XXIII University Hospital, IISPV, Universitat Rovira i Virgili, Tarragona, Spain. [Escoté,X; Ceperuelo-Mallafré,MV; Garrido-Sánchez,L; Miranda,M; Vendrell,J] CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain. [Pérez-Pérez,R; Peral,B] Instituto de Investigaciones Biomédicas, Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC) & Universidad Autónoma de Madrid (UAM), Madrid, Spain. [Fernández-Real,JM] Diabetes, Endocrinology and Nutrition Service, Institut d’Investigació Biomèdica de Girona (IdIBGi), Girona, Spain. [Tinahones Madueño,FJ] Servicio Endocrinología y Nutrición del Hospital Universitario Virgen de la Victoria, Málaga, Spain.
Palabras clave : Resistencia a la Insulina
Obesidad Mórbida
Obesidad
Proteínas de Unión a Ácidos Grasos
ARN Mensajero
Receptores Activados del Proliferador del Peroxisoma
Tejido Adiposo
Hígado
Experimentación Animal
Índice de Masa Corporal
Grasa Intraabdominal
Secuencia de Bases
Reacción en Cadena en Tiempo Real de la Polimerasa
Tejido Subcutáneo
MeSH: Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Hyperinsulinism::Insulin Resistance
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity::Obesity, Morbid
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding Proteins
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messenger
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Peroxisome Proliferator-Activated Receptors
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue
Medical Subject Headings::Anatomy::Digestive System::Liver
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Animal Experimentation
Medical Subject Headings::Health Care::Environment and Public Health::Public Health::Epidemiologic Measurements::Biometry::Anthropometry::Body Mass Index
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Abdominal Fat::Intra-Abdominal Fat
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Real-Time Polymerase Chain Reaction
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Subcutaneous Tissue
Fecha de publicación : 5-Nov-2012
Editorial : Public Library of Science
Cita Bibliográfica: Queipo-Ortuño MI, Escoté X, Ceperuelo-Mallafré MV, Garrido-Sanchez L, Miranda M, Clemente-Postigo M, et al. FABP4 Dynamics in Obesity: Discrepancies in Adipose Tissue and Liver Expression Regarding Circulating Plasma Levels. PLoS ONE. 2012; 7(11):e48605
Abstract: BACKGROUND FABP4 is predominantly expressed in adipose tissue, and its circulating levels are linked with obesity and a poor atherogenic profile. OBJECTIVE In patients with a wide BMI range, we analyze FABP4 expression in adipose and hepatic tissues in the settings of obesity and insulin resistance. Associations between FABP4 expression in adipose tissue and the FABP4 plasma level as well as the main adipogenic and lipolytic genes expressed in adipose tissue were also analyzed. METHODS The expression of several lipogenic, lipolytic, PPAR family and FABP family genes was analyzed by real time PCR. FABP4 protein expression in total adipose tissues and its fractions were determined by western blot. RESULTS In obesity FABP4 expression was down-regulated (at both mRNA and protein levels), with its levels mainly predicted by ATGL and inversely by the HOMA-IR index. The BMI appeared as the only determinant of the FABP4 variation in both adipose tissue depots. FABP4 plasma levels showed a significant progressive increase according to BMI but no association was detected between FABP4 circulating levels and SAT or VAT FABP4 gene expression. The gene expression of FABP1, FABP4 and FABP5 in hepatic tissue was significantly higher in tissue from the obese IR patients compared to the non-IR group. CONCLUSION The inverse pattern in FABP4 expression between adipose and hepatic tissue observed in morbid obese patients, regarding the IR context, suggests that both tissues may act in a balanced manner. These differences may help us to understand the discrepancies between circulating plasma levels and adipose tissue expression in obesity.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/845
Versión del editor : http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0048605
DOI: 10.1371/journal.pone.0048605
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Virgen de la Victoria

Files in This Item:
File Description SizeFormat 
Queipo-Ortuño_FABP4.pdfArtículo publicado331,92 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons