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Title: Mechanisms of hydrogen peroxide-induced vasoconstriction in the isolated perfused rat kidney.
Authors: Moreno, J M
Rodriguez Gomez, I
Wangensteen, R
Perez-Abud, R
Duarte, J
Osuna, A
Vargas, F
metadata.dc.contributor.authoraffiliation: [Moreno,JM; Rodriguez Gomez,I; Vargas,F] Departamento de Fisiología, Facultad de Medicina, Granada, Spain. [Wangensteen,R] Departamento de Ciencias de la Salud, Universidad de Jaen, Spain. [Perez-Abud,R; Osuna,A] Servicio de Nefrologia, Unidad Experimental, Hospital Virgen de las Nieves, Granada, Spain. [Duarte,J] Departamento de Farmacologia, Facultad de Farmacia, Granada, Spain.
Keywords: Hydrogen Peroxide;Catalase;Hydroxyl Radical;Kidney;Ca2+;Protein kinase C;Sexual Dimorphism;Renal Perfusion Pressure;Catalase;Vasoconstriction;Peróxido de Hidrógeno;Radical Hidroxilo;Riñón;Vasoconstricción
metadata.dc.subject.mesh: Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Catalase
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Free Radicals::Reactive Oxygen Species::Peroxides::Hydrogen Peroxide
Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Free Radicals::Reactive Oxygen Species::Hydroxyl Radical
Medical Subject Headings::Anatomy::Urogenital System::Urinary Tract::Kidney
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Sex Characteristics
Medical Subject Headings::Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Cardiovascular Physiological Phenomena::Cardiovascular Physiological Processes::Hemodynamics::Vasoconstriction
Medical Subject Headings::Organisms::Eukaryota::Animals
Issue Date: Jun-2010
Publisher: Krakow Polish Physiological Society
Citation: Moreno JM, Rodriguez Gomez I, Wangensteen R, Perez-Abud R, Duarte J, Osuna A, et al. Mechanisms of hydrogen peroxide-induced vasoconstriction in the isolated perfused rat kidney. J. Physiol. Pharmacol. 2010 ; 61(3):325-32
Abstract: The vasoconstrictor effect of hydrogen peroxide (H(2)O(2)) on isolated perfused rat kidney was investigated. H(2)O(2) induced vasoconstriction in the isolated rat kidney in a concentration-dependent manner. The vasoconstrictor effects of H(2)O(2) were completely inhibited by 1200 U/ml catalase. Endothelium-removal potentiated the renal response to H(2)O(2). The H(2)O(2) dose-response curve was not significantly modified by administration of the NO inhibitor L-NAME (10(-4) mol/l), whereas it was increased by the non-specific inhibitor of K+-channels, tetraethylammonium (3.10(-3) mol/l). Separately, removal of extracellular Ca(2+), administration of a mixture of calcium desensitizing agents (nitroprusside, papaverine, and diazoxide), and administration of a protein kinase C (PKC) inhibitor (chelerythrine, 10(-5) mol/l) each significantly attenuated the vasoconstrictor response to H(2)O(2), which was virtually suppressed when they were performed together. The pressor response to H(2)O(2) was not affected by: dimethyl sulfoxide (7.10(-5) mol/l) plus mannitol (3.10(-5) mol/l); intracellular Ca(2+) chelation using BAPTA (10(-5) mol/l); calcium store depletion after repeated doses of phenylephrine (10(-5) g/g kidney); or the presence of indomethacin (10(-5) mol/l), ODYA (2.10(-6) mol/l) or genistein (10(-5) mol/l). We conclude that the vasoconstrictor response to H(2)O(2) in the rat renal vasculature comprises the following components: 1) extracellular calcium influx, 2) activation of PKC, and 3) stimulation of pathways leading to sensitization of contractile elements to calcium. Moreover, a reduced pressor responsiveness to H(2)O(2) in female kidneys was observed.
Description: Journal Article; Research Support, Non-U.S. Gov't;
ISSN: 1899-1505 (Online)
0867-5910 (Print)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves

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