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Título : Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
Autor : Henn, Dominic
Bandner-Risch, Doris
Perttunen, Hilja
Schmied, Wolfram
Porras, Carlos
Ceballos, Francisco
RodrÍguez-Losada, Noela
Schäfers, Hans-Joachim
Filiación: [Henn,D; Bandner-Risch,D; Perttunen,H; Schmied,W; Schäfers,H-J] Department of Thoracic and Cardiovascular Surgery, Saarland University Hospital, Homburg/Saar, Germany. [Porras,C] Department of Cardiac Surgery, Hospital Universitario Virgen de la Victoria de Málaga, Spain. [Ceballos,F] Department of Genetics, Faculty of Biology, University of Santiago de Compostela, Spain. [Rodríguez-Losada,N] Foundation IMABIS, Carlos Haya University Hospital, Avenida Carlos Haya, Malaga, Spain.
Palabras clave : Aneurisma de la Aorta
Enfermedades de las Válvulas Cardíacas
Estudios de Asociación Genética
Expresión Génica
Factor 2B Eucariótico de Iniciación
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
MeSH: Medical Subject Headings::Diseases::Cardiovascular Diseases::Vascular Diseases::Aneurysm::Aortic Aneurysm
Medical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Heart Valve Diseases
Medical Subject Headings::Diseases::Cardiovascular Diseases::Vascular Diseases::Hypertension
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Genetic Association Studies
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Anatomy::Tissues
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::GTP-Binding Protein Regulators::Guanine Nucleotide Exchange Factors::Eukaryotic Initiation Factor-2B
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Check Tags::Male
Fecha de publicación : 11-Jan-2013
Editorial : Public Library of Science
Cita Bibliográfica: Henn D, Bandner-Risch D, Perttunen H, Schmied W, Porras C, Ceballos F, et al. Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms. PLoS ONE. 2013; 8(1):e54132
Abstract: Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aortic dilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascending aortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas were obtained from 60 patients (age ≤70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24, dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 had hypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperatively from the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RT-PCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32 genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the different specimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication with ACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, and PPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable for gene expression analysis of ascending aortic tissue.
Descripción : Journal Article;
Versión del editor :
DOI: 10.1371/journal.pone.0054132
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Regional de Málaga
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Virgen de la Victoria

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