Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/907
Título : Laboratory detection of respiratory viruses by automated techniques.
Autor : Pérez-Ruiz, Mercedes
Pedrosa-Corral, Irene
Sanbonmatsu-Gámez, Sara
Navarro-Marí, María
Filiación: [Pérez-Ruiz,M; Pedrosa-Corral,I; Sanbonmatsu-Gámez,S; Navarro-Marí,M] Laboratorio de Referencia de Salud Pública para Enfermedades con Sospecha de Etiología Vírica en Andalucía (Consejería de Salud), Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Granada, Spain.
Palabras clave : Acute respiratory infection
Automation
Influenza-like illness
microarray
Real-time PCR
Respiratory viruses
Infecciones del Sistema Respiratorio
Automatización
Análisis por Micromatrices
Reacción en Cadena en Tiempo Real de la Polimerasa
MeSH: Medical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract Infections
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Automation, Laboratory
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microchip Analytical Procedures::Microarray Analysis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Real-Time Polymerase Chain Reaction
Fecha de publicación : 30-Nov-2012
Editorial : Bentham Open
Cita Bibliográfica: Pérez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-Gámez S, Navarro-Marí M. Laboratory detection of respiratory viruses by automated techniques. Open Virol J 2009; 6:151-9
Abstract: Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.
Descripción : Journal Article; Se utiliza la url que lleva al articulo en pdf porque la url del abstract es incorrecta.
URI: http://hdl.handle.net/10668/907
Versión del editor : http://www.benthamscience.com/open/tovj/articles/V006/SI0103TOVJ/151TOVJ.pdf
DOI: 10.2174/1874357901206010151
ISSN : 1874-3579 (Online)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves

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