Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/916
Título : Gut microbiota in children with type 1 diabetes differs from that in healthy children: a case-control study.
Autor : Murri, Mora
Leiva, Isabel
Gómez-Zumaquero, Juan Miguel
Tinahones, Francisco J
Cardona, Fernando
Soriguer, Federico
Queipo-Ortuño, María Isabel
Filiación: [Murri,M; Cardona,F; Queipo-Ortuño,MI] Biomedical Research Laboratory, Hospital Universitario Virgen de la Victoria (FIMABIS), Málaga, Spain. [Leiva,I] Pediatric Endocrinology Service, Carlos Haya Materno Infantil Hospital, Málaga, Spain. [Gómez-Zumaquero,JM] Molecular Biology Laboratory, Civil Hospital (IMABIS foundation), Málaga, Spain. [Tinahones,FJ; Cardona,F; Queipo-Ortuño,MI] CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. [Soriguer,F] Endocrinology and Nutrition Service, Carlos Haya Hospital, Málaga, Spain. [Soriguer,F] CIBER of Diabetes and Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain. [Tinahones,FJ] Endocrinology and Nutrition Service, Hospital Universitario Virgen de la Victoria, Málaga, Spain.
Palabras clave : butyrate-producing bacteria
glycemic level
gut integrity
gut microbiota
gut permeability
HbA1c level
lactic acid-producing bacteria
mode of delivery
mucin
PCR-DGGE
type 1 diabetes
Permeabilidad
Ácido Láctico
Hemoglobina A Glucosilada
Niño
Intestino
Diabetes Mellitus Tipo 1
Ácido Butírico
MeSH: Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus::Diabetes Mellitus, Type 1
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Acids, Acyclic::Butyric Acids
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycosides::Hemoglobin A, Glycosylated
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Hydroxy Acids::Lactates::Lactic Acid
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Mucoproteins::Mucins
Medical Subject Headings::Organisms::Bacteria
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Bifidobacterium
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines
Medical Subject Headings::Named Groups::Persons::Age Groups::Child
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Permeability
Medical Subject Headings::Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Ecosystem::Biodiversity::Biota
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies
Fecha de publicación : Feb-2013
Editorial : BioMed Central
Cita Bibliográfica: Murri M, Leiva I, Gomez-Zumaquero JM, Tinahones FJ, Cardona F, Soriguer F, et al. Gut microbiota in children with type 1 diabetes differs from that in healthy children: a case-control study. BMC Med. 2013:11(1):46
Abstract: BACKGROUND A recent study using a rat model found significant differences at the time of diabetes onset in the bacterial communities responsible for type 1 diabetes modulation. We hypothesized that type 1 diabetes in humans could also be linked to a specific gut microbiota. Our aim was to quantify and evaluate the difference in the composition of gut microbiota between children with type 1 diabetes and healthy children and to determine the possible relationship of the gut microbiota of children with type 1 diabetes with the glycemic level. METHODS A case-control study was carried out with 16 children with type 1 diabetes and 16 healthy children. The fecal bacteria composition was investigated by polymerase chain reaction-denaturing gradient gel electrophoresis and real-time quantitative polymerase chain reaction. RESULTS The mean similarity index was 47.39% for the healthy children and 37.56% for the children with diabetes, whereas the intergroup similarity index was 26.69%. In the children with diabetes, the bacterial number of Actinobacteria and Firmicutes, and the Firmicutes to Bacteroidetes ratio were all significantly decreased, with the quantity of Bacteroidetes significantly increased with respect to healthy children. At the genus level, we found a significant increase in the number of Clostridium, Bacteroides and Veillonella and a significant decrease in the number of Lactobacillus, Bifidobacterium, Blautia coccoides/Eubacterium rectale group and Prevotella in the children with diabetes. We also found that the number of Bifidobacterium and Lactobacillus, and the Firmicutes to Bacteroidetes ratio correlated negatively and significantly with the plasma glucose level while the quantity of Clostridium correlated positively and significantly with the plasma glucose level in the diabetes group. CONCLUSIONS This is the first study showing that type 1 diabetes is associated with compositional changes in gut microbiota. The significant differences in the number of Bifidobacterium, Lactobacillus and Clostridium and in the Firmicutes to Bacteroidetes ratio observed between the two groups could be related to the glycemic level in the group with diabetes. Moreover, the quantity of bacteria essential to maintain gut integrity was significantly lower in the children with diabetes than the healthy children. These findings could be useful for developing strategies to control the development of type 1 diabetes by modifying the gut microbiota.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/916
Versión del editor : http://www.biomedcentral.com/1741-7015/11/46/abstract
DOI: 10.1186/1741-7015-11-46
ISSN : 1741-7015 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga
01- Artículos - Hospital Virgen de la Victoria

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