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Title: Detection of gluten immunogenic peptides in the urine of patients with coeliac disease reveals transgressions in the gluten-free diet and incomplete mucosal healing.
Authors: Moreno, María de Lourdes
Cebolla, Ángel
Muñoz-Suano, Alba
Carrillo-Carrion, Carolina
Comino, Isabel
Pizarro, Ángeles
León, Francisco
Rodríguez-Herrera, Alfonso
Sousa, Carolina
metadata.dc.subject.mesh: Adolescent
Antibodies, Monoclonal
Case-Control Studies
Celiac Disease
Child, Preschool
Chromatography, Affinity
Diet Records
Diet, Gluten-Free
GTP-Binding Proteins
Immunoglobulin A
Middle Aged
Patient Compliance
Protein Glutamine gamma Glutamyltransferase 2
Sensitivity and Specificity
Young Adult
Issue Date: 25-Nov-2015
Abstract: Gluten-free diet (GFD) is the only management for coeliac disease (CD). Available methods to assess GFD compliance are insufficiently sensitive to detect occasional dietary transgressions that may cause gut mucosal damage. We aimed to develop a method to determine gluten intake and monitor GFD compliance in patients with CD and to evaluate its correlation with mucosal damage. Urine samples of 76 healthy subjects and 58 patients with CD subjected to different gluten dietary conditions were collected. A lateral flow test (LFT) with the highly sensitive and specific G12 monoclonal antibody for the most dominant gluten immunogenic peptides (GIP) and a LFT reader were used to quantify GIP in solid-phase extracted urines. GIP were detectable in concentrated urines from healthy individuals previously subjected to GFD as early as 4-6 h after single gluten intake, and remained detectable for 1-2 days. The urine assay revealed infringement of the GFD in about 50% of the patients. Analysis of duodenal biopsies revealed that most of patients with CD (89%) with no villous atrophy had no detectable GIP in urine, while all patients with quantifiable GIP in urine showed incomplete intestinal mucosa recovery. GIP are detected in urine after gluten consumption, enabling a new and non-invasive method to monitor GFD compliance and transgressions. The method was sensitive, specific and simple enough to be convenient for clinical monitoring of patients with CD as well as for basic and clinical research applications including drug development. NCT02344758.
metadata.dc.identifier.doi: 10.1136/gutjnl-2015-310148
Appears in Collections:Producción 2020

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