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Title: Gene Expression Profiling Supports the Neural Crest Origin of Adult Rodent Carotid Body Stem Cells and Identifies CD10 as a Marker for Mesectoderm-Committed Progenitors.
Authors: Navarro-Guerrero, Elena
Platero-Luengo, Aida
Linares-Clemente, Pedro
Cases, Ildefonso
López-Barneo, José
Pardal, Ricardo
Keywords: Adult neural stem cells;Angiogenesis;CD10;Carotid body;Hypoxia;Neural crest
metadata.dc.subject.mesh: Animals
Carotid Body
Cell Count
Cell Differentiation
Cell Hypoxia
Cell Lineage
Gene Expression Profiling
Gene Expression Regulation
Mice, Transgenic
Neural Crest
Neural Stem Cells
Oligonucleotide Array Sequence Analysis
Rats, Wistar
Spheroids, Cellular
Stem Cell Niche
Issue Date: 2-Mar-2016
Abstract: Neural stem cells (NSCs) are promising tools for understanding nervous system plasticity and repair, but their use is hampered by the lack of markers suitable for their prospective isolation and characterization. The carotid body (CB) contains a population of peripheral NSCs, which support organ growth during acclimatization to hypoxia. We have set up CB neurosphere (NS) cultures enriched in differentiated neuronal (glomus) cells versus undifferentiated progenitors to investigate molecular hallmarks of cell classes within the CB stem cell (CBSC) niche. Microarray gene expression analysis in NS is compatible with CBSCs being neural crest derived-multipotent progenitor cells able to sustain CB growth upon exposure to hypoxia. Moreover, we have identified CD10 as a marker suitable for isolation of a population of CB mesectoderm-committed progenitor cells. CD10 + cells are resting in normoxia, and during hypoxia they are activated to proliferate and to eventually complete maturation into mesectodermal cells, thus participating in the angiogenesis necessary for CB growth. Our results shed light into the molecular and cellular mechanisms involved in CBSC fate choice, favoring a potential use of these cells for cell therapy. Stem Cells 2016;34:1637-1650.
metadata.dc.identifier.doi: 10.1002/stem.2331
Appears in Collections:Producción 2020

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