Please use this identifier to cite or link to this item:
Title: Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance.
Authors: Silva, Sonia
Altmannova, Veronika
Luke-Glaser, Sarah
Henriksen, Peter
Gallina, Irene
Yang, Xuejiao
Choudhary, Chunaram
Luke, Brian
Krejci, Lumir
Lisby, Michael
Keywords: DNA repair;Mph1;Mte1;genome integrity;homologous recombination;telomere maintenance
metadata.dc.subject.mesh: Crossing Over, Genetic
DEAD-box RNA Helicases
Gene Deletion
Protein Transport
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Stress, Physiological
Telomere Homeostasis
Telomere-Binding Proteins
Issue Date: 10-Mar-2016
Abstract: Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.
metadata.dc.identifier.doi: 10.1101/gad.276204.115
Appears in Collections:Producción 2020

Files in This Item:
File SizeFormat 
PMC4803055.pdf1,47 MBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons