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Title: Independent Candidate Serum Protein Biomarkers of Response to Adalimumab and to Infliximab in Rheumatoid Arthritis: An Exploratory Study.
Authors: Ortea, Ignacio
Roschitzki, Bernd
López-Rodríguez, Rosario
Tomero, Eva G
Ovalles, Juan G
López-Longo, Javier
de la Torre, Inmaculada
González-Alvaro, Isidoro
Gómez-Reino, Juan J
González, Antonio
metadata.dc.subject.mesh: Adalimumab
Antirheumatic Agents
Arthritis, Rheumatoid
Blood Proteins
Middle Aged
Issue Date: 6-Apr-2016
Abstract: Response to treatment of rheumatoid arthritis shows large inter-individual variability. This heterogeneity is observed with all the anti-rheumatic drugs, including the commonly used TNF inhibitors. It seems that drug-specific and target-specific factors lead individual patients to respond or not to a given drug, although this point has been challenged. The search of biomarkers distinguishing responders from non-responders has included shotgun proteomics of serum, as a previous study of response to infliximab, an anti-TNF antibody. Here, we have used the same study design and technology to search biomarkers of response to a different anti-TNF antibody, adalimumab, and we have compared the results obtained for the two anti-TNF drugs. Search of biomarkers of response to adalimumab included depletion of the most abundant serum proteins, 8-plex isobaric tag for relative and absolute quantitation (iTRAQ) labeling, two-dimensional liquid chromatography fractionation and relative quantification with a hybrid Orbitrap mass spectrometer. With this approach, 264 proteins were identified in all the samples with at least 2 peptides and 95% confidence. Nine proteins showed differences between non-responders and responders (P
metadata.dc.identifier.doi: 10.1371/journal.pone.0153140
Appears in Collections:Producción 2020

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