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Title: Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells.
Authors: Arellano-Orden, Elena
Calero-Acuña, Carmen
Moreno-Mata, Nicolás
Gómez-Izquierdo, Lourdes
Sánchez-López, Verónica
López-Ramírez, Cecilia
Tobar, Daniela
López-Villalobos, José Luis
Gutiérrez, Cesar
Blanco-Orozco, Ana
López-Campos, José Luis
metadata.dc.subject.mesh: Aged
Case-Control Studies
Cell Differentiation
Dendritic Cells
Flow Cytometry
Middle Aged
Myeloid Cells
Pulmonary Disease, Chronic Obstructive
Issue Date: 8-Apr-2016
Abstract: Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer. A total of 75 consecutive patients were included. Spirometry testing was used to identify COPD. Lung parenchyma sections anatomically distant from the primary lesion were examined. We used flow cytometry to identify different DCs subtypes-including BDCA1-positive myeloid DCs (mDCs), BDCA3-positive mDCs, and plasmacytoid DCs (pDCs)-and determine their maturation markers (CD40, CD80, CD83, and CD86) in all participants. We also identified follicular DCs (fDCs), Langerhans DCs (LDCs), and pDCs in 42 patients by immunohistochemistry. COPD was diagnosed in 43 patients (16 current smokers and 27 former smokers), whereas the remaining 32 subjects were classified as non-COPD (11 current smokers, 13 former smokers, and 8 never smokers). The number and maturation of DCs did not differ significantly between COPD and non-COPD patients. However, the results of flow cytometry indicated that maturation markers CD40 and CD83 of BDCA1-positive mDCs were significantly decreased in smokers than in non-smokers (P = 0.023 and 0.013, respectively). Immunohistochemistry also revealed a lower number of LDCs in COPD patients than in non-COPD subjects. Cigarette smoke, rather than airflow limitation, is the main determinant of impaired DCs maturation in the lung.
metadata.dc.identifier.doi: 10.1371/journal.pone.0152737
Appears in Collections:Producción 2020

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